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Biosensors available for kinetic and quantitation assays on Octet® systems

Biosensor   Description
Anti-hIgG Fc Capture (AHC) Immobilization of human IgG or other human Fc-containing proteins by binding to the human Fc region. Kinetic applications include protein and antibody kinetic screening, affinity characterization (ka, kd, KD), epitope binning, and hit validation.
Anti-hIgG Fc Capture for Quantitation (AHQ) Quantitation measurements of human IgG’s or human Fc-fusion proteins
Anti-Mouse Fc Capture (AMC) Binds the Fc portion of IgG1, IgG2a, IgG2b for capture-based immobilization. Applications include kinetic analysis of antibody-antigen interactions (ka, kd, KD) and off-rate screening. IgG3 should be evaluated on a case-by-case basis.
Anti-Mouse Fc Capture for quantitation (AMQ) Quantitation measurements of mouse IgG’s or mouse F(ab’)2
Anti-Human Fab-CH1 2nd generation (FAB2G) (FAB2 Binds specifically to the CH1 domain of human IgG, allowing quantitation and kinetic characterization of human Fab, F(ab’)2, and IgGs with no recognition towards free light chains.
Protein A (ProA) Protein A coated biosensor for capture and quantiation of IgG’s of various species
Protein G (ProG) Protein G coated biosensor for capture and quantitation of IgG’s of various species.
Portein L (ProL) Protein L coated biosensor for capture and quantitation of IgG’s of various species
Streptavidin (SA) Streptavidin-coated biosensor with high binding capacity. Immobilizes biotinylated antibodies, proteins, and nucleic acids to form a stable surface. Kinetic applications include protein and antibody kinetic screening, affinity characterization (ka, kd, KD), epitope binning, and hit validation.
High Precision Streptavidin (SAX) Streptavidin-coated biosensor for Immobilizing biotinylated molecules for high precision quantitation and kinetic measurements
High Precision Streptavidin 2.0 (SAX2) Streptavidin-coated biosensor with reproducible biotin-ligand loading from one lot to another. QC tested to limit biotinylated ligand loading variance, providing confident detection of variance from samples. Suited for product release QC, activity assays in manufacturing and other highly precise quantitation and kinetic applications
Super Streptavidin (SSA) Streptavidin-coated biosensor with a very high density of biotin binding sites. Immobilizes biotinylated proteins, peptides, nucleic acids and small molecules to form a stable surface. Kinetic applications include antibody, protein, peptide, and small molecule kinetic screening, affinity characterization (ka, kd, KD), epitope binning, and hit validation
Anti-GST (GST) High affinity anti-GST antibody enables direct and rapid quantitation of GST-tagged biomolecules and easy and reliable capture of GST-tagged biomolecules for rapid kinetic analysis.
Anti-Penta HIS (HIS1K) Capture of His-tagged proteins for kinetic analysis with target analytes. Quantitation of His-tagged proteins in buffer, media or diluted lysate. The sensor is pre-coated with Penta-His antibody from Qiagen
Anti-HIS (HIS2) Quantitation of HIS-tagged proteins in crude matrices or buffer or column eluent. The sensor is pre-coated with anti-His Ab from MBS.
Ni-NTA (Ni-NTA) Nickel charged tris-NTA with strong binding to HIS-tagged recombinant proteins for kinetic measurements and quantitation.
Amine Reactive Second Generation (AR2G) Second-generation carboxylate-functionalized surface allows covalent coupling of proteins via EDC/s-NHS mediated amide bond formation. The second-generation surface provides increased loading density, more robust loading conditions and decreased non-specific binding compared to the original AR Biosensor for most proteins. Kinetic applications include protein and antibody kinetic screening, affinity characterization (ka, kd, KD), and hit validation. Requires AR2G Assay Kit (part no. 18-5095).
Aminopropylsilane (APS) Adsorption of proteins and membrane fractions through hydrophobic moieties. Kinetic applications include adsorption of proteins and membrane fractions through hydrophobic moieties for kinetic screening and affinity characterization (ka, kd, KD).



 
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