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100 - 250ml ÀÏȸ¿ë vessel À» ÀÌ¿ëÇÏ´Â ÃֽŠ°³¹ßµÈ Çõ½ÅÀûÀÎ ¼º´ÉÀÇ
microbioreactor system |
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¹Ì»ý¹°¹è¾ç ¶Ç´Â cell culture ¿¡ »ç¿ë |
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ÇϳªÀÇ module ¿¡ ÀÏȸ¿ë vessel µÎ°³°¡ ÀåÂø |
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ÃÖ´ë 4°³ÀÇ module ÀÌ controller ¿¡ ¿¬°á °¡´ÉÇϹǷΠÃÑ 8°³ÀÇ bioreactor ¸¦ µ¿½Ã¿¡
¼¼Æ÷¹è¾ç °¡´É |
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Working Volume : 100 - 250ml |
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pitch blade impeller ¶Ç´Â rushton impeller »ç¿ë |
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Spot based DO (dissolved oxygen) ¼¾¼ |
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ÀÏȸ¿ë pH electrode |
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bioreactor ´ç 5°³ÀÇ liquid reservoir ³»Àå
- 2°³ÀÇ 125ml reservoir
- 3°³ÀÇ 50ml reservoir |
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°¢ reservoir ¿¡´Â syringe pump °¡ ÀåÂøµÇ¾î revervoir ÀÇ liquid ¸¦ Á¤¹ÐÇÏ°Ô vessel ·Î ÀÌ¼Û |
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liquid reservoir ¿ÜºÎ¿¡´Â ³Ã°¢ÀåÄ¡°¡ ÀÖ¾î ¿Âµµ¿¡ ¹Î°¨ÇÑ liquid ¸¦ »ç¿ëÇÒ °æ¿ì reservoir ÀÇ ¿Âµµ¸¦
6¡É ~ 8¡É ·Î À¯Áö °¡´É |
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°¢ bioreactor station ¿¡´Â peristaltic pump °¡ º°µµ·Î ÀåÂøµÇ¾î ÀÖ¾î vessel ¿¡ º°µµÀÇ liquid ¸¦ °ø±ÞÇϰųª (feeding) vessel ³»ºÎÀÇ media ¸¦
»Ì¾Æ ³»´Â (harvesting) ÀÛ¾÷À» ÇÒ ¼ö ÀÖ´Ù. |
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exhaust gas condenser ³»Àå |
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gas °¡ µé¾î¿À´Â inlet ºÎºÐ¿¡´Â filter ³»Àå |
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sparge gassing ¶Ç´Â headspace gassing Áß ¼±Åà |
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°¢ bioreactor ¿¡´Â 3Á¾·ùÀÇ gas ¸¦ °ø±Þ °¡´É
- cell culture : O2, CO2, N2 | Air
- microbial : O2, Air, N2 |
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°¢ gas °ø±ÞÇÏ´Â line ¿¡´Â °¢°¢ mass flow censor ÀåÂø |
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°³º°ÀûÀ¸·Î ÀÌ·ç¾îÁö´Â bioreactor vessel ÀÇ heating °ú cooling Á¶Àý |
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°¢ bioreactor vessel ³»ºÎÀÇ impeller ȸÀü ¼Óµµ´Â °³º°ÀûÀ¸·Î Á¶Àý |
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¹èÃâ°¡½ººÐ¼®ÀåÄ¡ÀÎ BioPAT ambr Xgas ¸¦ ¼±Åûç¾çÀ¸·Î ÀåÂøÇϸé bioreactor ·Î ºÎÅÍ ¹èÃâµÇ´Â O2, CO2 gas ¸¦ ºÐ¼®ÇÏ¿©
´ÙÀ½°ú °°Àº °á°ú¸¦ ºÐ¼® °¡´É.
- OUR (oxygen uptake rate) : »ê¼Ò ¼Òºñ ¼Óµµ °è»ê
- CER (Carbon Dioxide Evolution Rate) : ź¼Ò ¹ß»ý ¼Óµµ °è»ê |
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