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¿µÁøÄÚÆÛ·¹ÀÌ¼Ç Á¾ÇÕÄ«´Ù·Î±× |
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LUMiSpoc Single Particle Counter | |
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Module : Hydrodynamic Focusing |
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Two-dimensional signal intensities and distribution densities of scattered light in the forward and sideward direction | |
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Counting of Polystyrene-Mix (8 size classes) | |
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Counting of Polystyrene-Mix (8 size classes) | |
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Cat. No. |
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324-00000,   LUMiSpoc¢ç Single Particle Counter |
°ßÀû¿äû |
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LUMiSpoc¢ç Àº suspension °ú emulsion »ó¿¡ ÀÖ´Â ³ª³ë & ¸¶ÀÌÅ©·Î ÀÔÀÚ (¶Ç´Â droplet) ÀÇ
particle size distribution °ú particle concentration À» ÃøÁ¤ÇÏ´Â ÃÖ°í ¼º´ÉÀÇ single particle ºÐ¼®ÀåºñÀÌ´Ù. |
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ÀÌ Àåºñ°¡ °³º° ÀÔÀÚ¸¦ ÃøÁ¤ÇÏ´Â ¹æ½ÄÀº »õº° ¼¼Æ÷ÀÇ °³¼ö¸¦ ÃøÁ¤ÇÏ´Â flow cytometer ¿Í À¯»çÇÏ´Ù.
°³º° ÀÔÀÚ°¡ laser beam À» Åë°úÇÒ ¶§ ÀÔÀÚ¸¦ Åë°úÇÏ´Â Á÷Áø ¹æÇâÀ¸·Î scattering ÇÏ´Â forward light ¿Í
ÀÔÀÚ¿¡¼ 90µµ °¢µµ·Î ¹Ý»çÇÏ¿© scattering ÇÏ´Â sideward light ¸¦ ÃøÁ¤ÇÏ´Â ±â¼úÀ» ±â¹ÝÀ¸·Î Çϴµ¥
ÀÌ ±â¼úÀ» Single Particle Light-Scattering Technology¢ç À̶ó ÇÏ¸ç ¼¼°è ÃÖ°íÀÇ ÀÔÀÚ Æ¯¼º ºÐ¼® ±â¼úÀ» °¡Áö°í
ÀÖ´Â µ¶ÀÏ LUM »çÀÇ Æ¯Çã ¹ÞÀº ±â¼úÀÌ´Ù. |
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Single Particle Light-Scattering Technology¢ç (SPLS) |
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optical flow cell ¿¡¼ ºü¸£°Ô È帣°í ÀÖ´Â sheath flow ¿¡ ÀÇÇØ Áß¾Ó¿¡¼ õõÈ÷ È帣´Â sample flow ¿¡ ÀÖ´Â particle µéÀº
Çϳª¾¿ ¶³¾îÁ® (hydrodynamic focusing ) ·¹ÀÌÀúºö À§Ä¡·Î À̵¿ÇÏ°Ô µÈ´Ù. |
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single particle ÀÌ ·¹ÀÌÀúºöÀ» Åë°úÇÏ¸é ºûÀº ¸ðµç ¹æÇâÀ¸·Î scattering ÇÏ°Ô µÇ´Âµ¥ 0¡Æ ¹æÇâ (forward) °ú 90¡Æ ¹æÇâ (sideward) ¿¡¼
scattering light ¸¦ µ¿½Ã¿¡ ÁõÆø, ÃøÁ¤ÇÏ¿© ½Ç½Ã°£À¸·Î ºÐ¼®ÇÏ°Ô µÈ´Ù. |
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ÃøÁ¤µÈ °³º° ÀÔÀÚÀÇ scattered light intensity ´Â ¹é¸¸°³ ÀÌ»óÀÇ bin À¸·Î sorting µÇ¾î °íÇØ»ó bar Â÷Æ®·Î Ç¥½ÃµÇ¾î
particle ÀÇ count distribution À» ³ªÅ¸³»°Ô µÈ´Ù. |
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ºÐ·ùµÈ intensity ´Â Mie À̷п¡ ÀÇÇØ particle size distribution density ·Î ÀüȯµÇ¾î Ç¥½ÃµÈ´Ù. |
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Single Particle Counting : ÀÔÀÚ Çϳª ÇϳªÀÇ Å©±â¸¦ ÃøÁ¤ |
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ÃÖ¼Ò 50nm Å©±âÀÇ ÀÔÀÚ¿¡¼ ÃÖ´ë 8§ Å©±âÀÇ ÀÔÀÚ±îÁö ÃøÁ¤ °¡´É
- 40 nm to 350 nm (sideward scattering)
- 200 nm - 8,000 nm (forward scattering) |
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range º¯È¯ ¶Ç´Â component ±³Ã¼¾øÀÌ ³ÐÀº ¹üÀ§ÀÇ ÀÔÀÚ Å©±â¸¦ ÃøÁ¤ °¡´É |
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°³º° ÀÔÀÚÀÇ Å©±â¸¦ ÃøÁ¤ (sizing) ÇÏ°í °°Àº Å©±âÀÇ ÀÔÀÚ °³¼ö¸¦ °è»ê (counting) ÇÏ´Â ¹æ½Ä |
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diode laser beam À» ÀÌ¿ëÇÏ¿© ÀÔÀÚ Å©±â ÃøÁ¤ |
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sideward scattered light ¿Í forward scattered light ¸¦ µ¿½Ã¿¡ ÃøÁ¤ÇÏ¿© ÀÔÀÚÅ©±â ÃøÁ¤ |
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sideward scattered light ¿Í forward scattered light ´Â µÎ °³ÀÇ µ¿ÀÏÇÑ ±¤ÁõÆø±â¸¦ ÀÌ¿ëÇÏ¿©
light ½ÅÈ£¸¦ ÁõÆøÇϹǷΠ¸Å¿ì Á¤¹ÐÇÏ°í ½Å·Ú¼º ÀÖ´Â ÀÔÀÚ Å©±â µ¥ÀÌÅÍ Á¦°ø |
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µÎ °³ scattered light ÀÇ signal intensity ¿Í distribution density ¸¦ 2-D ±×·¡ÇÁ·Î Ç¥½Ã |
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15.6 ÀÎÄ¡ ÅÍÄ¡ µð½ºÇ÷¹ÀÌ¿¡ ÀÔÀÚ °³¼ö¿Í scatter intensity °¡ ½Ç½Ã°£À¸·Î Ç¥½Ã |
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ÃÖ¼Ò 15nm Â÷ÀÌ°¡ ³ª´Â Å©±âÀÇ ÀÔÀÚµéÀ» ±¸ºÐ °¡´É |
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·¹ÀÌÀúºöÀº ÃÖ´ë ÃÊ´ç 10,000 ¹ø±îÁö ÀÔÀÚÅ©±â ÃøÁ¤ °¡´É |
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250 §¡ (recommended) ±îÁö ÃÖ¼Ò·®ÀÇ »ùÇà »ç¿ë °¡´É |
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sample ÃøÁ¤ ½Ã°£Àº 5ºÐÀ¸·Î ¸Å¿ì ªÀº ½Ã°£¿¡ ÃøÁ¤ °¡´É |
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ÃøÁ¤ °¡´ÉÇÑ ÀÔÀÚ ³óµµ ¹üÀ§ : 106/ml ~ 109/ml |
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À¯¼¼Æ÷ ºÐ¼®±â¿¡ ³Î¸® »ç¿ëµÇ´Â ÃֽŠ±â´ÉÀÇ FCS Express 7 ¼ÒÇÁÆ®¿þ¾î¸¦ ÀåÂøÇÏ¿©
ÃøÁ¤µÈ raw data ¸¦ ÀǹÌÀÖ´Â ´Ù¾çÇÑ ¾ç½ÄÀ¸·Î ÀüȯÇÏ¿© Á¦°ø |
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Å©±â¸¦ ¾Ë°í ÀÖ´Â ÂüÁ¶ ÀÔÀÚ¸¦ ÀÌ¿ëÇÏ¿© one point calibration ÀÌ °¡´ÉÇϹǷΠcalibration ÀÌ ¸Å¿ì Æí¸®ÇÏ´Ù |
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sample ÃøÁ¤, ¼¼Ã´ µîÀÇ ÀÛ¾÷ÀÌ Àü ÀÚµ¿À¸·Î ÁøÇà |
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Applications
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Particle counting and size determination |
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Number concentration determination |
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Direct determination of the number-based particle size distribution of nano- and microparticles |
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Classification of nanomaterials |
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Recording of agglomeration & flocculation kinetics |
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Determination of distribution tails |
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Particle contamination detection |
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Separation membrane & filter medium testing, cut-off determination |
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Dispersions of: CMP slurries, carbon black, pigments, filler, pharmaceutical emulsions & suspensions,
reference & smart particles, biological cells, viruses |
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Specification of LUMiSpoc Single Particle Counter |
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Particle Size Range |
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40 nm to 350 nm (sideward scattering)
200 nm - 8,000 nm (forward scattering) |
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Min. Size Resolution (x < 1 ¥ìm) |
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< 15 nm |
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Particle Concentration Range |
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106/ml to 109/ml |
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Counting Rate |
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up to 10,000 per second |
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Typical Measurement Time |
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5 min |
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Light Source |
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Diode laser, 405 nm, 40 - 100 mW (adjustable) |
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Particle Counting Precision |
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< 2 % |
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Sample Volume |
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as small as 250 ¥ìl (recommended) |
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Dimensions (W x H x D) |
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60 x 52 x 59 cm3 |
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Weight |
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25kg |
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Power supply |
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24 V DC, 220W |
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