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Vero Cell 은 live 또는 inactivated viral vaccine 생산에 가장 널리 사용되어 온 mammalian cell line |
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4Cell® NutriVero™ Flex 10 media 는 Vero Cell 을 이용하여 바이러스 백신 개발 및 생산을 할 때 사용하는 Vero Cell 배양 media |
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Vero Cell 의 성장과 influenza virus 의 expansion 목적으로 널리 사용 |
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100% Chemically Defined (CD), serum-free, protein-free, animal component-free, 그리고 hydrolysate-free 배양액 |
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Vero Cell 은 2D monolayer 또는 3D microcarrier suspension 방식으로 세포를 배양 |
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백신 및 다른 바이오의약품 생산을 위한 다양한 cGMP 규정에 부합되는 media |
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어떤 동물성 성분도 포함하고 있지 않으며 또한 어떠한 식물성 또는 효모 추출물 과 펩톤성분도 포함하고 있지 않은 Media |
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세포 배양 효율과 바이러스 생산 효율이 매우 탁월한 media |
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다양한 배양 조건에서도 항상 일정한 세포 성장능력과 바이러스 생산능력을 항상 일정하게 유지합니다. |
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이 media 하나로서 Vero cell 의 seeding, growth, virus infection 전 과정을 모두 진행 가능 |
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bench scale 부터 production scale 까지 모두 가능 |
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serum-free media 이므로 downstream 과정인 purification, filtration 같은 과정이 매우 편리하게 진행됩니다. |
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Media 에 Hydrolysates 가 없으므로 항상 일정하고 동일한 배양 결과를 구할 수 있습니다. |
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100% chemically defined medium 으로 제조되어 동물성 성분으로 인한 contamination 가능성이 없습니다. |
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ISO 13408 과 cGMP guideline 에 적합하게 제조된 media |
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대량의 세포배양에도 효율적으로 사용 |
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■ 4Cell® NutriVero™ Flex 10 Performance in Vero Cell 2D Culturing System |
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| Note :: Vero cells were seeded in T25 flasks at a cell density of 40,000 cells/cm² and
incubated at 37 ºC in a humidified atmosphere and 5% CO₂ with 4Cell® NutriVero™ Flex 10 or reference medium. |
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■ Vero Cell Growth Density in a 3D Culture System |
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| Note :: Two parallel bioreactors were filled up to 2 L of working volume of
chemically defined 4Cell® NutriVero™ Flex 10 and undefined reference medium. Stirring speed was set
between 70 and 130 rpm, temperature set to 37 ºC and pH controlled to 7.2.
The bioreactors were seeded with 0.15×109 cells/L and 3 g/L of microcarriers. |
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■ Initial Assessment of Defined 4Cell® NutriVero™ Flex 10 Medium Viral Production Capacity |
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| Note :: Vero cells were seeded in 6-well plates at a cell density of 30,000 cells/cm² of culture,
the cultures were infected with various viruses: Measles, Sabin poliovirus type 1 and EV71-C4. Following 7 days the cultures
showed a positive CPE and the supernatant was harvested and analyzed for the amount of infectious particles by means of a virus titration procedure. |
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■ Enterovirus 71-C4 Virus Production in a 3D Microcarriers Culture System |
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| Note :: Vero cells were cultured in 1 L bioreactors at 0.12±0.03×106 cells/mL for 3 days,
up to a concentration of 0.9±0.2×106 cells/mL. At 66 hours post seeding, the cells were infected with EV71-C4 at a titer of 7.55 log10 TCID 50/mL.
Samples were taken at indicated time points post infection, and analyzed for virus titer. |
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| Description |
Form |
Package |
Volume |
Order Number |
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| 4Cell® NutriVero™ Flex 10 Medium |
Liquid |
Bottle |
0.5 L |
CFV3FA4009 |
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| 4Cell® NutriVero™ Flex 10 Medium |
Liquid |
Bottle |
1 L |
CFV3FA4010 |
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| 4Cell® NutriVero™ Flex 10 Medium P-Red Medium |
Liquid |
Bottle |
0.5 L |
CFV3FA4000 |
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| 4Cell® NutriVero™ Flex 10 Medium P-Red Medium |
Liquid |
Bottle |
1 L |
CFV3FA4001 |
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| 4Cell® NutriVero™ 10 L Bag |
Liquid |
Bag |
10 L |
CFV3FA4013 |
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| 4Cell® NutriVero™ 20 L Bag |
Liquid |
Bag |
20 L |
CFV3FA4014 |
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| Description |
Form |
Package |
Volume |
Order Number |
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| 4Cell® NutriVero™ Powder and Supplement 10 L |
Powder and frozen supplement |
Bucket and bottle |
10 L |
CQV3FA5002 |
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| 4Cell® NutriVero™ Powder and Supplement 50 L |
Powder and frozen supplement |
Bucket and bottle |
50 L |
CQV3FA5003 |
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| 4Cell® NutriVero™ Powder and Supplement 100 L |
Powder and frozen supplement |
Bucket and bottle |
100 L |
CQV3FA5004 |
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