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¿µÁøÄÚÆÛ·¹ÀÌ¼Ç Á¾ÇÕÄ«´Ù·Î±×
Optimal Culture of hPSCs (Human Pluripotent Stem Cell) for Research and Cell Therapies
 
- NutriStem¢ç hPSC XF Medium (scientific reference)
- Improved Cellular Maintenance of Human Pluripotent Stem Cells (poster)
- NutriStem¢ç hPSC XF Medium Datasheet (datasheet)
- From Cells to Organs - the Organoid Stepping-Stone (white paper)
- NutriStem¢ç hPSC XF Manual (manual)


 
 
¡î Áٱ⼼Æ÷ ¿¬±¸¸ðµ¨À» Àç»ý Áٱ⼼Æ÷ Ä¡·á¿Í °°Àº ÀÓ»óÀûÀ¸·Î »ç¿ëÇϱâ À§Çؼ­´Â °íÇ°ÁúÀÇ ¼¼Æ÷¹è¾ç ½Ã½ºÅÛ°ú ÀûÀýÇÑ documentation ÀÌ ÇÊ¿äÇÕ´Ï´Ù. Sartorius ´Â human induced pluripotent stem cells (hiPSCs) ¿Í human embryonic stem cells (hESCs) ÀÇ ¼¼Æ÷ ¹è¾çÀ» À§ÇÑ ÃÖÀûÀÇ È¯°æÀ¸·Î¼­ NutriStem¢ç serum-free, xeno-free media ¿Í °ü·Ã ½Ã¾àÀ» °ø±ÞÇÏ°í ÀÖ½À´Ï´Ù.
¡î NutriStem¢ç media ¿Í °ü·Ã ½Ã¾àµéÀº ¸ðµÎ cGMP ±ÔÁ¤¿¡ ÀûÇÕÇÑ ½Ã¼³¿¡¼­ ¸Å¿ì ¾ÈÀüÇÏ°Ô Á¦Á¶µÇ°í ÀÖ½À´Ï´Ù.
¡î medium ³»ÀÇ bFGF (basic fibroblast growth factor) ³óµµ¸¦ ÃÖ¼Ò·Î ÇÏ¿© Áٱ⼼Æ÷ÀÇ ºÐÈ­ÀÇ ÀûÀýÇÑ À¯µµ¿¡ µµ¿òÀÌ µÇµµ·Ï ÇÕ´Ï´Ù.
¡î ´Ù¸¥ stimulatory growth factor ¶Ç´Â ´Ù¸¥ Cytokine ¾øÀÌ human pluripotent cells ¸¦ ¹è¾çÇÒ ¼ö ÀÖ´Â medium ÀÔ´Ï´Ù.
¡î 100% Chemically Defined (CD), Xeno-free and Serum-free ¹è¾ç¾× ÀÔ´Ï´Ù.
¡î human iPSCs ¿Í hESCs ¼¼Æ÷ÀÇ ÃÖÀû ¹è¾çÀ» À§ÇØ ¼³°èµÈ medium ÀÔ´Ï´Ù.
¡î ¿øÀç·á, ¾ÈÀü¼º µ¥ÀÌÅÍ, È¿´É µ¥ÀÌÅÍ µî°ú °ü·ÃµÈ Á¤º¸ÀÎ Drug Master File (DMF) Á¦°øÀÌ °¡´ÉÇÕ´Ï´Ù.
¡î Feeder ¾ø´Â ȯ°æ¿¡¼­ ¼¼Æ÷ ¹è¾ç
¡î hES ¿Í hiPS ¼¼Æ÷ÀÇ ¹è¾ç¿¡ ÇʼöÀûÀÎ ±Ø¼Ò·®ÀÇ protein ¸¸À» Æ÷ÇÔÇÏ°í ÀÖ½À´Ï´Ù.
¡î Ç×»ó ÀÏÁ¤ÇÑ ¼¼Æ÷¹è¾ç ¼º´ÉÀ» Á¦°øÇÏ¸ç ¶ÇÇÑ Ç×»ó ¿¹Ãø °¡´ÉÇÑ cellular behavior °¡ ÀÌ·ç¾îÁöµµ·Ï ÇÕ´Ï´Ù.
¡î 50 passages ÀÌ»óÀÇ long-term culture °¡ °¡´ÉÇϸç ÀÌ °æ¿ì¿¡µµ ¼¼Æ÷ ¹è¾ç ÀçÇö¼ºÀÌ Å¹¿ùÇÕ´Ï´Ù.
¡î ¼¼Æ÷ÀÇ attachment ´É·Â°ú proliferation rate °¡ ¸Å¿ì Ź¿ùÇϹǷΠhigh-throughput screening application ¿¡ ÀûÇÕÇÑ ¹è¾ç¾×ÀÔ´Ï´Ù.
¡î NutriStem¢ç hPSC XF Medium ¿¡¼­ ¹è¾çµÈ ¼¼Æ÷´Â °Ç°­ÇÑ ¹ÌºÐÈ­ hES and hiPS ¼¼Æ÷µéÀÌ °¡Áö´Â ÇüÅÂÀû Ư¡ÀÎ ¨ç Å« Nucleus-to-Cytoplasm ºñÀ², ¨è È®¿¬ÇÑ Nucleoli, ±×¸®°í ¨é ¼±¸íÇÑ Colony °æ°è¸é °ú °°Àº Ư¡À» Çö¹Ì°æÀ¸·Î ¼Õ½±°Ô °üÂûÇÒ ¼ö ÀÖ½À´Ï´Ù.
¡î Human pluripotent stem cells Àº endoderm, mesoderm, and ectoderm °ú °°Àº 3°³ÀÇ germ layer ·Î ºÐÈ­Çϴ Ư¼ºÀ» °¡Áö°í Àִµ¥ NutriStem¢ç hPSC XF Medium ¿¡¼­ ¹è¾çµÈ ¼¼Æ÷´Â embryoid body ³»¿¡¼­ ÀÚ¿¬ÀûÀ¸·Î ÀÌ·¯ÇÑ ºÐÈ­°¡ ÀϾ°í ÀÖ´Ù´Â °ÍÀ» È®ÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù.
¡î NutriStem¢ç hPSC XF (growth factor free) medium (Cat. # 06-5100-01-1A) Àº ¸Å¿ì º¹ÀâÇÑ ¼ººÐÀ¸·Î ±¸¼ºµÈ medium À¸·Î¼­ induced pluripotent stem (iPS) cell colony »ý¼º°ú °°Àº ÀÛ¾÷À» ÇϱâÀü¿¡ ªÀº ±â°£µ¿¾È ¼¼Æ÷¸¦ ¹è¾çÇϴµ¥ »ç¿ëÇÏ´Â medium ÀÔ´Ï´Ù.
¡î NutriStem¢ç hPSC XF (growth factor free) medium ¿¡¼­ ¹è¾çÇÑ iPSC line Àº Á¤»óÀûÀÎ NutriStem¢ç hPSC XF medium ¿¡ ¿Å°Ü expansion °ú long-term maintenance ¸ñÀûÀ¸·Î ¹è¾çÇÏ°Ô µË´Ï´Ù.



Peformance Test

¡á NutriStem¢ç hPSC XF Medium enables excellent proliferation of undifferentiated hES and hiPS cells
Note :: Proliferation of H1 hES cells cultured in Matrigel-coated 96-well plates in NutriStem¢ç hPSC XF Medium and the leading competing medium for feeder-free culture. Medium was changed and proliferation was assessed every 24 hours in culture.


¡á Normal Colony Morphology
Note :: H1 hES cells (panel A) and ACS1014 hiPS cells (panel B) cultured in NutriStem¢ç hPSC XF Medium on Matrigel-coated plates display colony morphologies typical of normal feeder-free hES and hiPS cell cultures, including a uniform colony of tightly compacted cells and distinct colony edges


¡á Embryoid Body Formation
Note :: Embryoid bodies (EBs) were generated from H9.2 hES cells cultured for 16 passages in NutriStem¢ç hPSC XF Medium on Matrigel matrix as an evaluation of pluripotency. The pluripotent H9.2 cells were suspended in serum-supplemented medium, where they spontaneously formed EBs containing cells of embryonic germ layers. The following cell types were identified by examination of the histological sections of 14-day-old EBs stained with H&E: (A) neural rosette (ectoderm), (B) neural rosette stained with Tubulin, (C) primitive blood vessels (mesoderm), and (D) megakaryocytes (mesoderm).


¡á Taratoma Formation
Note :: H9.2 hES cells were cultured for 11 passages in NutriStem¢ç hPSC XF Medium using a human foreskin fibroblast (HFF) feeder layer. The hES cells were subsequently injected into the hind leg muscle of SCID-beige mice for in vitro evaluation of pluripotency. The following tissues from all three germ layers were identified in H&E-stained histological sections of the teratoma 12 weeks post-injection: (A) cartilage (mesoderm), (B) epithelium (endoderm), and (C) neural rosette (ectoderm).



Ordering Information
Product Name Order Number Size Storage
NutriStem¢ç hPSC XF Medium 05-100-1A 500 ml -20 ¡ÆC
05-100-1B 100 ml
NutriStem¢ç hPSC XF Medium
(Modified, GF-free, bFGF-free)
06-5100-01-1A 500 ml -20 ¡ÆC
NutriStem¢ç hPSC XF with +20 ng/ml bFGF 06-5100-11-1A 500 ml -20 ¡ÆC
LaminStem¢â 521 05-753-1 1 ml -20 ¡ÆC
Vitronectin ACF 05-754-0002 1 ml -20 ¡ÆC to -80 ¡ÆC
Recombinant Trypsin Solution 03-078-1A 500 ml RT
03-078-1B 100 ml
Recombinant Trypsin EDTA Solution 03-079-1A 500 ml RT
03-079-1B 100 ml
EDTA Solution 0.5M 01-862-1B 100 ml RT
Accutase Solution 03-073-1B 100 ml -20 ¡ÆC
NutriFreez¢ç D10 Cryopreservation Medium 05-713-1A 500 ml 2-8 ¡ÆC
05-713-1B 100 ml
05-713-1C 20 ml
05-713-1D 10 ml
05-713-1E 50 ml



 
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