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Robust and Effective Base Medium for Infection and Stable Expression
 
- HEK Media and Feed Portfolio (brochure)
- Protocol HEK GM Media (protocol)
- The Journey to AAV Production in Suspension - Scaling-UP Your Process (application note)
- Navigating Upstream AAV Production for Gene Therapy Manufacturing (white paper)


 
 
¡î complete chemically defined, animal-component-free and hydrolysate-free medium
¡î suspension culture ¿¡¼­ recombinant proteins °ú antibodies »ý»êÀ» À§ÇÑ ¼¼Æ÷ ¹è¾ç¿¡ ÃÖ°íÀÇ È¿À²À» º¸ÀÌ´Â culture media
¡î HEK293 ¼¼Æ÷¿Í ´Ù¸¥ human cell lines ¿¡ ´ëÇÑ infection È¿À²¼ºÀÌ ¸Å¿ì Ź¿ùÇÑ media
¡î Áß°£ Á¤µµ level ÀÇ nutrients °ú growth factors ÇÔ·®À» °¡Áö°í ÀÖ´Â media
¡î thawing, expanding, pre-complexing DNA, transfecting ±×¸®°í producing °ú °°Àº °ÅÀÇ ¸ðµç ´Ü°è¿¡¼­ µ¿ÀÏÇÑ media ·Î »ç¿ë°¡´ÉÇÑ media
¡î ´Ù¸¥ serum-free media ¿¡¼­ ¹è¾çÇÑ ¼¼Æ÷µµ adaptation °úÁ¤ ÇÊ¿ä¾øÀÌ ¹è¾ç °¡´ÉÇÑ media
¡î seed train culture ¿¡¼­ suspension cell ÀÇ ¼ºÀåÀ» ¾ÈÁ¤ÀûÀ¸·Î À¯Áö½ÃÄÑÁÖ´Â media
¡î w/ HT (hypoxanthine/thymidine)
¡î w/ growth factor
¡î w/o L-glutamine
¡î DIN EN ISO 9001 ¿Í DIN EN ISO 13485 ¿¡ ÀÇÇØ Quality ½ÂÀÎ



Ordering Information
 
HEK GM Media
Media Description Format | Quantity | Package Order Number
HEK GM Robust and effective : Selected for stable expression with HEK cells Liquid | 1 L Bottle 851-0001
Liquid | 10 L Bag 851-0010BAG
Liquid | 20 L Bag 851-0020BAG
Liquid | 50 L Bag 851-0050BAG
Powder & Supplement | 10 L Container CQV3FB4002
Powder & Supplement | 100 L Container CQV3FB4004


HEK Media Sample Kits
Media Description Format | Quantity | Package Order Number
HEK293 Media Sample Kit To select the best fit media for the application and the feed Liquid | 4¡¿1 L Bottle CFV3FB4000



Technical Specifications
 
Attribute Growth
Factor
Hypoxanthine
/ Thymidine
Characteristics
/ Comparison
Production Methods
HEK GM With With ¡á Stable/Robust
¡á High nutrient level
¡á Infection
¡á Stable expression
HEK ViP NX Without With ¡á Highest nutrient level ¡á Transient transfection
¡á Infection
¡á Stable expression
HEK ViP NB Without With ¡á Medium nutrient level
¡á Leanest formulation
  (lowest number of components)
¡á Transient transfection
¡á Infection
¡á Stable expression
HEK TF With With ¡á Broad application ¡á Transient transfection
¡á Infection
¡á Stable expression
HEK FS_2 Feed Without With ¡á Feed Supplement to boost the productivity of the HEK media ¡á Transient transfection (use from 4-6 h post-transfection)
¡á Infection
¡á Stable expression



 
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