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Optimized for virus production : Best choice for transient expression in HEK cells
 
- HEK Media and Feed Portfolio (brochure)
- HEK ViP Media Family (flyer)
- Protocol HEK ViP NX Media (protocol)
- The Journey to AAV Production in Suspension - Scaling-UP Your Process (application note)
- Navigating Upstream AAV Production for Gene Therapy Manufacturing (white paper)


 
 
complete chemically defined, animal-component-free 그리고 hydrolysate-free medium
가장 풍부한 수준의 영양분으로 구성된 media
HEK 또는 다른 mammalian cell line 을 배양하여 AAV (adeno-associated virus) 와 lentivirus 를 transfection 하고 이 virus 와 viral vecor 의 생산 조건을 최적화 하는 목적으로 개발된 media
AAV 또는 lentivirus 를 이용하는 gene therapy 연구 및 생산에 매우 중요한 media
이 media 는 특히 PEI (polyethylenimine) 와 같은 polycationic transfection reagents 를 사용하는 transient transfection 에 매우 효과적인 media
다양한 용량의 suspension culture 에서 세포를 배양하고 proteins 또는 viral vectors 를 생산하는데 사용하는 media
다른 serum-free media 에서 배양한 세포도 adaptation 과정 필요없이 배양 가능한 media
seed train culture 에서 suspension cell 을 높은 viability 로 안정적으로 배양할 수 있는 media
virus 또는 viral vector 의 생산에 최적화 된 media
thawing, expanding, pre-complexing DNA, transfecting 그리고 producing 과 같은 거의 모든 단계에서 동일한 media 로 사용가능한 media
w/ HT (hypoxanthine/thymidine)
w/o growth factor
w/o L-glutamine
w/o phenol red
DIN EN ISO 9001 와 DIN EN ISO 13485 에 의해 Quality 승인



Ordering Information
 
HEK ViP NX Media
Media Description Format | Quantity | Package Order Number
HEK ViP NX Optimized for virus production : Best choice for transient expression in HEK cells Liquid | 1 L Bottle 892-0001
Liquid | 10 L Bag 892-0010BAG
Liquid | 20 L Bag 892-0020BAG
Liquid | 50 L Bag 892-0050BAG
Powder | 10 L Container 892-0010DPM
Powder | 100 L Container 892-0100DPM


HEK Media Sample Kits
Media Description Format | Quantity | Package Order Number
HEK293 Media Sample Kit To select the best fit media for the application and the feed Liquid | 4×1 L Bottle CFV3FB4000



Peformance Test

Figure 1 : AAV-2 titer boost : compatible with different HEK cell lines
 
Note. Relative genomic titers (qPCR) in culture supernatant of different suspension HEK cell lines in their respective reference media compared to HEK ViP NX media.


Figure 2 : AAV-2 titer boost with different transfection reagents
Note. : Comparison of AAV-2 titers in supernatant vs. cells/pellets for processes with Xell's HEK ViP NX medium and two transfection reagents.


Figure 3 : AAV-2 titer boost by process optimization
Note. : Optimization of processes leads to increased titer in culture supernatant.


Figure 4 : AAV-8 Genomic Titer in Supernatant
 
Note. Comparison of AAV8 genomic titers in supernatants of HEK293 cells cultivated in Xell's HEK ViP NX medium. Cultivation in bioreactor leads to increased genomic titer in culture supernatant.



Technical Specifications
 
Attribute Growth
Factor
Hypoxanthine
/ Thymidine
Characteristics
/ Comparison
Production Methods
HEK GM With With Stable/Robust
High nutrient level
Infection
Stable expression
HEK ViP NX Without With Highest nutrient level Transient transfection
Infection
Stable expression
HEK ViP NB Without With Medium nutrient level
Leanest formulation
  (lowest number of components)
Transient transfection
Infection
Stable expression
HEK TF With With Broad application Transient transfection
Infection
Stable expression
HEK FS_2 Feed Without With Feed Supplement to boost the productivity of the HEK media Transient transfection (use from 4-6 h post-transfection)
Infection
Stable expression



 
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