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¿µÁøÄÚÆÛ·¹ÀÌ¼Ç Á¾ÇÕÄ«´Ù·Î±× |
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[Brochure] Apoptosis, Cytotoxicity, Mitochondrial
Membrane Potential and ATP Metabolism ºê·Î¼Å ¿øº»
[Application Note] Incucyte Apoptosis Assays for Kinetic Quantitation of Apoptosis
Incucyte¢ç non-perturbing cell health reagents ´Â ¿ÜºÎ¿¡¼ ÁÖ¾îÁö´Â ¾î¶² Àڱؿ¡ ´ëÇÑ Àå±â°£¿¡ °ÉÄ£ ¼¼Æ÷ ƯÀÌÀû ¹ÝÀÀÀ» ´Ù¾çÇÑ ÇüÅÂÀÇ °á°ú°ªÀ¸·Î º¸¿©ÁÝ´Ï´Ù. |
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¼¼Æ÷ ¼ºÀå¿¡ Àå¾Ö¸¦ ÀÏÀ¸Å°Áö ¾ÊÀ¸¸ç ¸ê±Õó¸®µÈ live-cell reagent ¸¦ »ç¿ëÇϹǷΠ¿À·ù°¡ ¾ø´Â »ç½Ç ±×´ë·ÎÀÇ »ý¹°ÇÐÀû ¹ÝÀÀÀ» º¸¿©ÁÝ´Ï´Ù. |
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mix-and-read reagents ¶Ç´Â genetically encoded reagents ±×¸®°í ÃÖÀûÀÇ protocol À» ÀÌ¿ëÇÏ¿© ¼ÒÁßÇÑ ¼¼Æ÷ÀÇ ¼Õ½ÇÀ» ÃÖ¼ÒÈ ÇÏ¿´½À´Ï´Ù. |
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single well ³»¿¡¼ cell health reagent ¸¦ ÀÌ¿ëÇÏ¿© ¿©·¯°¡Áö ºÐ¼®ÀÌ °¡´ÉÇϹǷΠdzºÎÇÑ ½ÇÇè µ¥ÀÌÅ͸¦ ±¸ÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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HD (high density) phase À̹ÌÁö¸¦ ÀÌ¿ëÇÏ¿© ¸Å ¼ø°£ÀÇ cell morphology º¯È¸¦ °üÂûÇÒ ¼ö ÀÖ½À´Ï´Ù. |
Application Spotlight : Incucyte¢ç Apoptosis Assay |
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apoptotic pathway analysis ¸¦ À§ÇÑ 2°³ÀÇ µ¶Æ¯ÇÑ Assay Kit ¸¦ ÀÌ¿ëÇÏ¿© Cell Death ¸¦ È®ÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù.
¨ç Incucyte¢ç Caspase-3/7 Dyes ¸¦ ÀÌ¿ëÇÏ¿© caspase-3/7 activation À» Á÷Á¢ÀûÀ¸·Î È®ÀÎÇϰųª
¨è Incucyte¢ç Annexin V Dyes ¸¦ ÀÌ¿ëÇÏ¿© apoptotic cells ³»ÀÇ ¹ßÇöµÈ phosphatidylserine À» ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù.
Á×Àº ¼¼Æ÷ÀÇ °³¼ö¸¦ counting ÇÑ °á°ú¸¦ ´Ù¾çÇÑ ÇüÅ·ΠȮÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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