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¿µÁøÄÚÆÛ·¹ÀÌ¼Ç Á¾ÇÕÄ«´Ù·Î±× |
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4Cell¢ç SmartCHO media ´Â ÃÖ½ÅÀÇ ¼¼Æ÷°øÇÐ ±â¼ú¿¡ ÀÇÇØ ÃÖ±Ù »õ·ÎÀÌ °³¹ßµÈ media ·Î¼ ¸ðµç Á¾·ùÀÇ CHO cell line ¿¡
»ç¿ëÇÒ ¼ö ÀÖ´Â °¡Àå Ź¿ùÇÑ ¼¼Æ÷ ¹è¾ç ¼º´ÉÀ» °¡Áø media & feed system ÀÔ´Ï´Ù.
fed-batch application ¿ëÀ¸·Î »ç¿ëµÇ´Â 4Cell¢ç SmartCHO media ´Â ´Ù¾çÇÑ Á¾·ùÀÇ CHO cell line ¿¡ ´ëÇØ Å×½ºÆ®¸¦ ÁøÇàÇÑ °á°ú
¸ðµç CHO cell line ÀÇ ¼ºÀå¿¡ Ź¿ùÇÑ ¼º´ÉÀ» º¸¿´À¸¸ç ¶ÇÇÑ mAB, bi-specifics, ±×¸®°í Fc fusion proteins ¿Í °°Àº product ÀÇ
»ý»ê È¿À²ÀÌ ¸Å¿ì ¿ì¼öÇÏ¿´½À´Ï´Ù.
CHO-GD44 cell line À» ÀÌ¿ëÇÏ¿© 4Cell¢ç SmartCHO media ¿Í ´Ù¸¥ À¯¸í ºê·£µå media ÀÇ fed-batch cilture performance ¸¦
ºñ±³ÇÏ´Â Å×½ºÆ®¸¦ ÁøÇàÇÏ¿´½À´Ï´Ù. ¶ÇÇÑ CHO-S, CHO-K1, CHO-GS cell line µµ Ãß°¡·Î ÀÌ ºñ±³Å×½ºÆ®¿¡ »ç¿ëÇÏ¿´À¸¸ç
¼¼Æ÷¹è¾çÀº Sartorius ÀÇ Ambr 15 microbioreactor ¸¦ ÀÌ¿ëÇÏ¿´½À´Ï´Ù.
Å×½ºÆ® °á°ú 4Cell¢ç SmartCHO media ´Â ´Ù¸¥ À¯¸í ºê·£µå media º¸´Ù CHO cell ¹è¾ç ´É·ÂÀÌ ´õ ¿ì¼öÇÏ´Ù´Â °ÍÀ» È®ÀÎÇÒ ¼ö ÀÖ¾ú½À´Ï´Ù.
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Ordering Information |
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4Cell¢ç SmartCHO |
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Media |
Description |
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Format | Quantity | Package |
Order Number |
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4Cell¢ç SmartCHO SAM |
Allows the cultivation or media adaptation of CHO cells |
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Liquid | 2¡¿1 L | Bottles |
CFP3FB1106 |
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Powder | 10 L | Container |
CQP3FB1146 |
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Powder | 25 L | Container |
CQP3FB1147 |
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Powder | 250 L | Container |
CQP3FB1148 |
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4Cell¢ç SmartCHO PM |
Non-selective production medium for batch or for fed batch culture. It can be
used for effective cultivation of any CHO cell lines, and helps produce high yield
of the active protein |
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Liquid | 2¡¿1 L | Bottles |
CFP3FB2107 |
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Powder | 9 L | Container |
CQP3FB2149 |
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Powder | 20 L | Container |
CQP3FB2150 |
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Powder | 200 L | Container |
CQP3FB2151 |
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4Cell¢ç SmartCHO FMA |
Ideal companion feeds designed to be used together with 4Cell¢ç
SmartCHO Production Medium |
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Liquid | 2¡¿1 L | Bottles |
CFP3FB3108 |
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Powder | 3 L | Container |
CQP3FB3152 |
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Powder | 30 L | Container |
CQP3FB3153 |
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4Cell¢ç SmartCHO FMB |
Ideal companion feeds designed to be used together with 4Cell¢ç
SmartCHO Production Medium |
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Liquid | 2¡¿125 mL | Bottles |
CFP3FB4109 |
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Liquid | 2¡¿500 mL | Bottles |
CFP3FB4110 |
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Powder | 2 L | Container |
CQP3FB4154 |
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Powder | 4 L | Container |
CQP3FB4155 |
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Powder | 45 L | Container |
CQP3FB4156 |
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Product Media |
Media |
Format | Quantity | Package |
Order Number |
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4Cell¢ç SmartCHO Media System |
Stock & Adaptation Medium (SAM) |
Liquid | 1 L | Bottle |
CFP3FB0200 |
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Production Medium (PM) |
Liquid | 2¡¿1 L | Bottles |
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Feed Medium A (FMA) |
Liquid | 1 L | Bottle |
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Feed Medium B (FMB) |
Liquid | 125 ml | Bottle |
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¾Æ·¡ Figure 1 °ú Figure 2 ´Â 4Cell¢ç SmartCHO media ÀÇ CHO cell line À» ¹è¾çÇÏ´Â ´É·ÂÀÌ
´Ù¸¥ À¯¸í ºê·£µå media ¿¡ ºñÇØ ÀϺκÐÀº À¯»çÇÏÁö¸¸ ´ëºÎºÐ ÈξÀ ´õ Ź¿ùÇÏ´Ù´Â ½ÇÇè °á°ú¸¦ º¸¿©ÁÖ°í ÀÖ½À´Ï´Ù. |
Figure 1 : Growth and Viability Profiles of Two CHO Clones in 4Cell ¢ç SmartCHO vs. Two Representative Commercial Media |
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Note. (A & B) CHO-S ¿Í (C & D) CHO-DG44 cells. ¼¼Æ÷´Â Å×½ºÆ® Çϱâ Àü 4½Ã°£µ¿¾È media ¿¡¼ ÀûÀÀ±â°£À» °¡Á³½À´Ï´Ù.
(A & C) Viable cell count (VCC) ¿Í (B & D) percentage viability ´Â 14Àϵ¿¾È ÃøÁ¤ÇÏ¿´½À´Ï´Ù. |
Figure 2 : Relative Growth of Three CHO Clones in Cultured in 4Cell ¢ç SmartCHO vs. Five Commercial Media |
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Note. (A) CHO-S, (B) Clone 1 CHO-DG44, (C) Clone 2 CHO-DG44.
¼¼Æ÷ÀÇ »ó´ë ¼ºÀå·üÀº IVCC (integrated viable cell concentration) ¹æ¹ýÀ¸·Î °è»êÇÏ¿´½À´Ï´Ù. |
Figure 3 : Titer and Specific Productivity of CHO Clones Cultured in 4Cell ¢ç SmartCHO vs. Commercial Media |
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Figure 3 Àº CHO ¼¼Æ÷ÀÇ Æ¯Á¤ »ý»ê¼ºÀº Ŭ·Ð°ú ¹èÁö¿¡ µû¶ó º¯µ¿¼ºÀÌ ¸Å¿ì Å«µ¥ ÀÌ´Â
cell line °ú ÀÀ¿ë ºÐ¾ß¿¡ µû¶ó ´Ù¾çÇÑ formulations, feeds, ±×¸®°í parameter µéÀ» Á¶»çÇÒ Çʿ伺ÀÌ ÀÖ´Ù´Â °ÍÀ» À̾߱âÇÏ°í ÀÖ½À´Ï´Ù. |
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Note. (A-C) Titer [%]; (D-F) Specific Productivity Rate [%]. (A+D) CHO-S, (B+E) Clone 1 CHO-DG44, (C+F) Clone 2 CHO-DG44 were cultured in
4Cell¢ç SmartCHO and five commercial media |
Figure 4 : : Product Aggregation in CHO Cells Cultured in 4Cell ¢ç SmartCHO vs. Commercial Media. |
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Note. Product aggregation determined by size exclusion chromatography
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cell culture media ´Â ¼¼Æ÷°¡ »ý»êÇÏ´Â product ÀÇ Ç°Áú¿¡ ¸Å¿ì Å« ¿µÇâÀ» ¹ÌÄ£´Ù.
Size-exclusion chromatography Å×½ºÆ® °á°ú CHO-S ¼¼Æ÷´Â ¾à°£ÀÇ Â÷ÀÌ´Â ÀÖÁö¸¸
media ¿Í »ó°ü¾øÀÌ ¸Å¿ì ³·Àº ¼öÁØÀÇ low aggregate values ¸¦ À¯ÁöÇÏ¿´½À´Ï´Ù.
CHO-DG44 (clone 1) ÀÇ °æ¿ì¿¡´Â 4Cell¢ç SmartCHO media ¿¡¼ °¡Àå ³·Àº
aggregate values ¸¦ ³ªÅ¸³»¾ú½À´Ï´Ù. |
Figure 5 : 4Cell ¢ç SmartCHO Media Supports Robust Growth of Two CHO Clones in 5 L Bioreactor Cultures. |
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Cell culture media ÀÇ ¼º´ÉÀº ´ë¿ë·® ¹è¿ë¿¡¼µµ ÀÏÁ¤ÇÏ¿©¾ß ÇÕ´Ï´Ù. Figure 5 ÀÇ µ¥ÀÌÅÍ¿¡¼ ¿ì¸®´Â 4Cell¢ç SmartCHO media ¸¦ 5 ¸®ÅÍ bioreactor ¿¡¼ÀÇ ¹è¾ç¿¡ »ç¿ëÇÑ °á°ú
¼¼Æ÷ ¹è¾ç °á°ú°¡ ¼Ò¿ë·®¶§¿Í µ¿ÀÏÇÏ°í ¹è¾çµµ ¸Å¿ì Ź¿ùÇÏ¿´´Ù´Â °ÍÀ» ¾Ë ¼ö ÀÖ½À´Ï´Ù. |
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Note. (A) CHO-S and (B) CHO-DG44 cells. Cells were adapted to the media for four weeks before testing. VCC = viable cell count |
Figure 6 : Productivity of CHO-S and CHO-DG44 Cell Grown in 4Cell ¢ç SmartCHO vs. Commercial Media. |
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4Cell¢ç SmartCHO media ´Â ³ôÀº ¼¼Æ÷ ƯÀ̼ºÀ» °¡Áø high-titer Ç×ü¸¦ °íÈ¿À²·Î »ý»êÇÒ ¼ö ÀÖ°Ô ÇÕ´Ï´Ù. |
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Note. (A) titer and (B) specific productivity. |
Figure 7 : Product Quality Attributes From CHO Cell Cultures Grown in 4Cell ¢ç SmartCHO vs. Commercial Media |
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Note. (A) aggregates and (B) charge variants (CHO-S cells only). |
Technical Specification |
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Media |
Surfactant |
Phenol Red |
L-Glutamine |
Hypoxanthine/ Thymidine |
Growth Factor |
Glucose |
Amino Acids |
Vitamins |
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SmartCHO¢ç (SAM|PM) |
¡á |
- |
- |
- |
- |
¡á |
¡á |
¡á |
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SmartCHO¢ç FMB |
- |
- |
- |
- |
- |
- |
¡á |
- |
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SmartCHO¢ç FMA |
¡á |
- |
- |
- |
- |
¡á |
¡á |
¡á |
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TCXD6 |
¡á |
- |
- |
¡á |
¡á |
¡á |
¡á |
¡á |
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TC-42 w/IGF |
¡á |
- |
- |
- |
¡á |
¡á |
¡á |
¡á |
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TC-42 w/rInsulin |
¡á |
- |
- |
- |
- |
¡á |
¡á |
¡á |
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TC-42 w/o GF |
¡á |
- |
- |
- |
- |
¡á |
¡á |
¡á |
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TCX10D |
¡á |
- |
- |
¡á |
- |
¡á |
¡á |
¡á |
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CHOlean |
¡á |
- |
- |
- |
- |
¡á |
¡á |
¡á |
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CHO TF |
¡á |
- |
- |
¡á |
- |
¡á |
¡á |
¡á |
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TCX7D feed |
¡á |
- |
- |
¡á |
- |
60 g/L |
¡á |
¡á |
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Basic Feed |
¡á |
- |
- |
¡á |
- |
20 g/L |
¡á |
¡á |
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