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¿µÁøÄÚÆÛ·¹ÀÌ¼Ç Á¾ÇÕÄ«´Ù·Î±×
Advanced, High-Performing CHO Media for all Types of CHO Cells
 
- 4Cell¢ç CHO Media and Feed Portfolio (brochure)
- Cell Culture Media and Buffer (brochure)


4Cell¢ç SmartCHO media ´Â ÃÖ½ÅÀÇ ¼¼Æ÷°øÇÐ ±â¼ú¿¡ ÀÇÇØ ÃÖ±Ù »õ·ÎÀÌ °³¹ßµÈ media ·Î¼­ ¸ðµç Á¾·ùÀÇ CHO cell line ¿¡ »ç¿ëÇÒ ¼ö ÀÖ´Â °¡Àå Ź¿ùÇÑ ¼¼Æ÷ ¹è¾ç ¼º´ÉÀ» °¡Áø media & feed system ÀÔ´Ï´Ù.

fed-batch application ¿ëÀ¸·Î »ç¿ëµÇ´Â 4Cell¢ç SmartCHO media ´Â ´Ù¾çÇÑ Á¾·ùÀÇ CHO cell line ¿¡ ´ëÇØ Å×½ºÆ®¸¦ ÁøÇàÇÑ °á°ú ¸ðµç CHO cell line ÀÇ ¼ºÀå¿¡ Ź¿ùÇÑ ¼º´ÉÀ» º¸¿´À¸¸ç ¶ÇÇÑ mAB, bi-specifics, ±×¸®°í Fc fusion proteins ¿Í °°Àº product ÀÇ »ý»ê È¿À²ÀÌ ¸Å¿ì ¿ì¼öÇÏ¿´½À´Ï´Ù.

CHO-GD44 cell line À» ÀÌ¿ëÇÏ¿© 4Cell¢ç SmartCHO media ¿Í ´Ù¸¥ À¯¸í ºê·£µå media ÀÇ fed-batch cilture performance ¸¦ ºñ±³ÇÏ´Â Å×½ºÆ®¸¦ ÁøÇàÇÏ¿´½À´Ï´Ù. ¶ÇÇÑ CHO-S, CHO-K1, CHO-GS cell line µµ Ãß°¡·Î ÀÌ ºñ±³Å×½ºÆ®¿¡ »ç¿ëÇÏ¿´À¸¸ç ¼¼Æ÷¹è¾çÀº Sartorius ÀÇ Ambr 15 microbioreactor ¸¦ ÀÌ¿ëÇÏ¿´½À´Ï´Ù.

Å×½ºÆ® °á°ú 4Cell¢ç SmartCHO media ´Â ´Ù¸¥ À¯¸í ºê·£µå media º¸´Ù CHO cell ¹è¾ç ´É·ÂÀÌ ´õ ¿ì¼öÇÏ´Ù´Â °ÍÀ» È®ÀÎÇÒ ¼ö ÀÖ¾ú½À´Ï´Ù.



Ordering Information
 
4Cell¢ç SmartCHO
Media Description Format | Quantity | Package Order Number
4Cell¢ç SmartCHO SAM Allows the cultivation or media adaptation of CHO cells Liquid | 2¡¿1 L | Bottles CFP3FB1106
Powder | 10 L | Container CQP3FB1146
Powder | 25 L | Container CQP3FB1147
Powder | 250 L | Container CQP3FB1148
4Cell¢ç SmartCHO PM Non-selective production medium for batch or for fed batch culture. It can be used for effective cultivation of any CHO cell lines, and helps produce high yield of the active protein Liquid | 2¡¿1 L | Bottles CFP3FB2107
Powder | 9 L | Container CQP3FB2149
Powder | 20 L | Container CQP3FB2150
Powder | 200 L | Container CQP3FB2151
4Cell¢ç SmartCHO FMA Ideal companion feeds designed to be used together with 4Cell¢ç SmartCHO Production Medium Liquid | 2¡¿1 L | Bottles CFP3FB3108
Powder | 3 L | Container CQP3FB3152
Powder | 30 L | Container CQP3FB3153
4Cell¢ç SmartCHO FMB Ideal companion feeds designed to be used together with 4Cell¢ç SmartCHO Production Medium Liquid | 2¡¿125 mL | Bottles CFP3FB4109
Liquid | 2¡¿500 mL | Bottles CFP3FB4110
Powder | 2 L | Container CQP3FB4154
Powder | 4 L | Container CQP3FB4155
Powder | 45 L | Container CQP3FB4156


CHO Media Sample Kits
Product Media Media Format | Quantity | Package Order Number
4Cell¢ç SmartCHO
Media System
Stock & Adaptation Medium (SAM) Liquid | 1 L | Bottle CFP3FB0200
Production Medium (PM) Liquid | 2¡¿1 L | Bottles
Feed Medium A (FMA) Liquid | 1 L | Bottle
Feed Medium B (FMB) Liquid | 125 ml | Bottle



Peformance Test


¾Æ·¡ Figure 1 °ú Figure 2 ´Â 4Cell¢ç SmartCHO media ÀÇ CHO cell line À» ¹è¾çÇÏ´Â ´É·ÂÀÌ ´Ù¸¥ À¯¸í ºê·£µå media ¿¡ ºñÇØ ÀϺκÐÀº À¯»çÇÏÁö¸¸ ´ëºÎºÐ ÈξÀ ´õ Ź¿ùÇÏ´Ù´Â ½ÇÇè °á°ú¸¦ º¸¿©ÁÖ°í ÀÖ½À´Ï´Ù.

Figure 1 : Growth and Viability Profiles of Two CHO Clones in 4Cell ¢ç SmartCHO vs. Two Representative Commercial Media
 
Note. (A & B) CHO-S ¿Í (C & D) CHO-DG44 cells. ¼¼Æ÷´Â Å×½ºÆ® Çϱâ Àü 4½Ã°£µ¿¾È media ¿¡¼­ ÀûÀÀ±â°£À» °¡Á³½À´Ï´Ù. (A & C) Viable cell count (VCC) ¿Í (B & D) percentage viability ´Â 14Àϵ¿¾È ÃøÁ¤ÇÏ¿´½À´Ï´Ù.

Figure 2 : Relative Growth of Three CHO Clones in Cultured in 4Cell ¢ç SmartCHO vs. Five Commercial Media
 
Note. (A) CHO-S, (B) Clone 1 CHO-DG44, (C) Clone 2 CHO-DG44. ¼¼Æ÷ÀÇ »ó´ë ¼ºÀå·üÀº IVCC (integrated viable cell concentration) ¹æ¹ýÀ¸·Î °è»êÇÏ¿´½À´Ï´Ù.



Figure 3 : Titer and Specific Productivity of CHO Clones Cultured in 4Cell ¢ç SmartCHO vs. Commercial Media
 
Figure 3 Àº CHO ¼¼Æ÷ÀÇ Æ¯Á¤ »ý»ê¼ºÀº Ŭ·Ð°ú ¹èÁö¿¡ µû¶ó º¯µ¿¼ºÀÌ ¸Å¿ì Å«µ¥ ÀÌ´Â cell line °ú ÀÀ¿ë ºÐ¾ß¿¡ µû¶ó ´Ù¾çÇÑ formulations, feeds, ±×¸®°í parameter µéÀ» Á¶»çÇÒ Çʿ伺ÀÌ ÀÖ´Ù´Â °ÍÀ» À̾߱âÇÏ°í ÀÖ½À´Ï´Ù.
 
Note. (A-C) Titer [%]; (D-F) Specific Productivity Rate [%]. (A+D) CHO-S, (B+E) Clone 1 CHO-DG44, (C+F) Clone 2 CHO-DG44 were cultured in 4Cell¢ç SmartCHO and five commercial media



Figure 4 : : Product Aggregation in CHO Cells Cultured in 4Cell ¢ç SmartCHO vs. Commercial Media.
 

Note. Product aggregation determined by size exclusion chromatography

cell culture media ´Â ¼¼Æ÷°¡ »ý»êÇÏ´Â product ÀÇ Ç°Áú¿¡ ¸Å¿ì Å« ¿µÇâÀ» ¹ÌÄ£´Ù. Size-exclusion chromatography Å×½ºÆ® °á°ú CHO-S ¼¼Æ÷´Â ¾à°£ÀÇ Â÷ÀÌ´Â ÀÖÁö¸¸ media ¿Í »ó°ü¾øÀÌ ¸Å¿ì ³·Àº ¼öÁØÀÇ low aggregate values ¸¦ À¯ÁöÇÏ¿´½À´Ï´Ù. CHO-DG44 (clone 1) ÀÇ °æ¿ì¿¡´Â 4Cell¢ç SmartCHO media ¿¡¼­ °¡Àå ³·Àº aggregate values ¸¦ ³ªÅ¸³»¾ú½À´Ï´Ù.



Figure 5 : 4Cell ¢ç SmartCHO Media Supports Robust Growth of Two CHO Clones in 5 L Bioreactor Cultures.
 
Cell culture media ÀÇ ¼º´ÉÀº ´ë¿ë·® ¹è¿ë¿¡¼­µµ ÀÏÁ¤ÇÏ¿©¾ß ÇÕ´Ï´Ù. Figure 5 ÀÇ µ¥ÀÌÅÍ¿¡¼­ ¿ì¸®´Â 4Cell¢ç SmartCHO media ¸¦ 5 ¸®ÅÍ bioreactor ¿¡¼­ÀÇ ¹è¾ç¿¡ »ç¿ëÇÑ °á°ú ¼¼Æ÷ ¹è¾ç °á°ú°¡ ¼Ò¿ë·®¶§¿Í µ¿ÀÏÇÏ°í ¹è¾çµµ ¸Å¿ì Ź¿ùÇÏ¿´´Ù´Â °ÍÀ» ¾Ë ¼ö ÀÖ½À´Ï´Ù.
 
Note. (A) CHO-S and (B) CHO-DG44 cells. Cells were adapted to the media for four weeks before testing. VCC = viable cell count



Figure 6 : Productivity of CHO-S and CHO-DG44 Cell Grown in 4Cell ¢ç SmartCHO vs. Commercial Media.
 
4Cell¢ç SmartCHO media ´Â ³ôÀº ¼¼Æ÷ ƯÀ̼ºÀ» °¡Áø high-titer Ç×ü¸¦ °íÈ¿À²·Î »ý»êÇÒ ¼ö ÀÖ°Ô ÇÕ´Ï´Ù.
 
Note. (A) titer and (B) specific productivity.



Figure 7 : Product Quality Attributes From CHO Cell Cultures Grown in 4Cell ¢ç SmartCHO vs. Commercial Media
 
Note. (A) aggregates and (B) charge variants (CHO-S cells only).



Technical Specification
 
Media Surfactant Phenol Red L-Glutamine Hypoxanthine/
Thymidine
Growth
Factor
Glucose Amino Acids Vitamins
SmartCHO¢ç (SAM|PM) ¡á - - - - ¡á ¡á ¡á
SmartCHO¢ç FMB - - - - - - ¡á -
SmartCHO¢ç FMA ¡á - - - - ¡á ¡á ¡á
TCXD6 ¡á - - ¡á ¡á ¡á ¡á ¡á
TC-42 w/IGF ¡á - - - ¡á ¡á ¡á ¡á
TC-42 w/rInsulin ¡á - - - - ¡á ¡á ¡á
TC-42 w/o GF ¡á - - - - ¡á ¡á ¡á
TCX10D ¡á - - ¡á - ¡á ¡á ¡á
CHOlean ¡á - - - - ¡á ¡á ¡á
CHO TF ¡á - - ¡á - ¡á ¡á ¡á
TCX7D feed ¡á - - ¡á - 60 g/L ¡á ¡á
Basic Feed ¡á - - ¡á - 20 g/L ¡á ¡á



 
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