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[Brochure] Antibody Internalization and Immunocytochemistry
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Incucyte¢ç antibody labeling reagents ´Â Çü±¤À¸·Î labeling µÇ¾îÁø Fab ·Î¼ ´Ù¾çÇÑ Fc-containg Ç×ü¿Í mixing ÇÏ¿© »ç¿ë °¡´ÉÇÏ´Ù.
ÀÌ reagent ¸¦ ¼¼Æ÷ ¹è¾çÁßÀÎ vessel ¿¡ ³Ö¾î °üÂûÇϸé fluorescently labeled Fabs °¡ ¼¼Æ÷³»·Î µé¾î°¡´Â °ÍÀ» È®ÀÎ °¡´ÉÇÕ´Ï´Ù. |
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¸ñÀûÀ¸·Î ÇÏ´Â Fc-containg Ç×ü¿Í single-step À¸·Î labeling ÀÌ °¡´ÉÇÏ°í ¶ÇÇÑ ¹Ù·Î ±× °á°ú¸¦ È®ÀÎ °¡´ÉÇϹǷΠ½ÇÇè ¿¬±¸ÀÇ »ý»ê¼ºÀÌ ¸Å¿ì Ź¿ùÇØÁý´Ï´Ù. |
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¼¼Æ÷ Ç¥¸é¿¡¼ÀÇ protein expression ¶Ç´Â antibody internalization ÀÇ º¯È¸¦ ½Ã°£¿¡ µû¸¥ cell function °ú morphology ¿Í ¿¬°ü½ÃÄÑ »ý°¢ÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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¸ðµç microplate well ¿¡¼´Â ÀϾ°í ÀÖ´Â protein dynamics ¸¦ ¾ÆÁÖ Á¤¹ÐÇÑ Çü±¤À¸·Î ÃøÁ¤ÇÑ ³»¿ëÀ» À̹ÌÁö¿Í ¿µ»óÀ» ÅëÇØ ½Ã°¢ÀûÀ¸·Î È®ÀÎÀÌ °¡´ÉÇÕ´Ï´Ù. |
Application Spotlight : Monitoring Dynamic Cell Surface Protein Expression |
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Incucyte¢ç Fabfluor-488 ¶Ç´Â Fabfluor-594 Antibody Labeling Reagents ¸¦ ÀÌ¿ëÇÏ¸é »ì¾ÆÀÖ´Â ¼¼Æ÷¿¡¼ ÁøÇàµÇ°í ÀÖ´Â cell surface protein expression À» Á¤·®È ÇÒ ¼ö ÀÖÀ¸¸ç
À̸¦ ÀÌ¿ëÇÏ¿© Àå±â°£¿¡ °ÉÄ£ ÀϾ°í ÀÖ´Â protein dynamics ¿Í function and morphology »çÀÌÀÇ ¿¬°ü¼ºÀ» È®ÀÎÇÏ´Â °Íµµ °¡´ÉÇÕ´Ï´Ù. |
Application Spotlight : Antibody Internalization |
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pH º¯È¿¡ µû¶ó Çü±¤ ¹ß±¤ÀÌ ´Þ¶óÁö´Â Ư¼ºÀ» °¡Áø Incucyte¢ç Fabfluor-pH Antibody Labeling Reagents ¸¦ ÀÌ¿ëÇϸé
»ý¸®Àû Á¶°Ç¿¡ µû¸¥ internalization rates ÀÇ ½Ç½Ã°£ ºÐ¼®À» ÅëÇØ antibody internalization Àüü °úÁ¤À» È¿°úÀûÀ¸·Î È®ÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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