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¿µÁøÄÚÆÛ·¹ÀÌ¼Ç Á¾ÇÕÄ«´Ù·Î±×
Well performing and robust medium for high-density cultivation and production with MDCK and MDBK cells
 
- 4Cell¢ç MDXK Medium (leaflet)
- 4Cell¢ç MDXK Medium Protocol (protocol)
- 4Cell¢ç MDXK Medium MSDS (safety data sheet)
- Solubilization of 4Cell¢ç MDXK powder (protocol)


 
 
¡î 4Cell¢ç MDXK Medium Àº ¹ÙÀÌ·¯½º °¨¿°ÀÌ ½±°Ô ÀϾ´Â MDCK/ MDBK (Madin-Darby Canine/Bovine Kidney) cell À» ¹è¾çÇϴµ¥ »ç¿ëÇÏ´Â ¹è¾ç¾×
¡î »ç¶÷¿¡ °¨¿°À» ÀÏÀ¸Å°´Â »õ·Î¿î influenza ¹ÙÀÌ·¯½º¿¡ ´ëÇÑ vaccine °³¹ß¿¡ ¸¹ÀÌ »ç¿ëµÇ´Â media
¡î MDXK cell ÀÇ ¼ºÀå°ú influenza virus ÀÇ expansion ¸ñÀûÀ¸·Î ³Î¸® »ç¿ë
¡î 100% Chemically Defined (CD), serum-free, protein-free, animal component-free, ±×¸®°í hydrolysate-free ¹è¾ç¾×
¡î human serum ¾øÀÌ »ç¿ëÇÏ´Â media ·Î¼­ serum ÀÌ ¾ø¾îµµ Ź¿ùÇÑ ¼¼Æ÷ ¹è¾ç ¼º´ÉÀ» ³ªÅ¸³¿
¡î MDXK cell ÀÇ ¹è¾ç°ú Influenza virus (H1N1, H3N2) ÀÇ expansion ÀÌ ÇϳªÀÇ media ·Î °¡´É
¡î ÀÌ media Çϳª·Î¼­ MDXK cell ÀÇ seeding, growth, virus infection Àü °úÁ¤À» ¸ðµÎ ÁøÇà °¡´É
¡î bench scale ºÎÅÍ production scale ±îÁö ¸ðµÎ °¡´É
¡î serum-free media À̹ǷΠdownstream °úÁ¤ÀÎ purification, filtration °°Àº °úÁ¤ÀÌ ¸Å¿ì Æí¸®ÇÏ°Ô ÁøÇàµË´Ï´Ù.
¡î WHO (World Health Organization) °¡ influenza A ¿Í influenza B viruses ÀÇ isolation ¿¡ °¡Àå ÀûÇÕÇÑ media ·Î ÃßõÇÑ Á¦Ç°ÀÔ´Ï´Ù.
¡î shaker flasks, spinner bottles ¶Ç´Â stirred tank bioreactors ¿¡¼­µµ influenza virus ÀÇ »ý»êÀÌ °íÈ¿À²·Î ÀÌ·ç¾î Áý´Ï´Ù.
¡î Media ¿¡ Hydrolysates °¡ ¾øÀ¸¹Ç·Î Ç×»ó ÀÏÁ¤ÇÏ°í µ¿ÀÏÇÑ ¹è¾ç °á°ú¸¦ ±¸ÇÒ ¼ö ÀÖ½À´Ï´Ù.
¡î 100% chemically defined medium À¸·Î Á¦Á¶µÇ¾î µ¿¹°¼º ¼ººÐÀ¸·Î ÀÎÇÑ contamination °¡´É¼ºÀÌ ¾ø½À´Ï´Ù.
¡î ISO 13408 °ú cGMP guideline ¿¡ ÀûÇÕÇÏ°Ô Á¦Á¶µÈ media
¡î ¿¬±¸ ¶Ç´Â ÀÓ»ó ºÐ¾ß ¸ðµÎ »ç¿ë °¡´É
¡î ´ë·®ÀÇ ¼¼Æ÷¹è¾ç¿¡µµ È¿À²ÀûÀ¸·Î »ç¿ë



Peformance Test

¡á MDXK Cell Growth in 4Cell¢ç MDXK Medium
Note :: 4Cell¢ç MDXK Medium performance have been evaluated on MDXK cells¡¯ growth in suspension


¡á 4Cell¢ç MDXK Medium Performance for Virus Production
Influenza Virus Infectious Titer (TCID50/mL) MOI TOI
A/Puerto Rico/8/34 H1N1 1.33E+07 10E-03 72H
A/Hong Kong/8/36 H3N2 8.32E+05 10E-02 72H
Note :: Virus quantification was performed by calculating the TCID50 (50% Tissue Culture Infective Dose) index. 4Cell¢ç MDXK Medium showed high abilities in supporting MDXK infection and virus productivity. Additionally to the TCID50 assay, ddPCR was performed for the samples A/Puerto Rico/8/34 (72 hours post-infection) to detect the total viral genome.
Total viral genome detection 4Cell¢ç MDXK Medium = 2.57E+07 copies/mL



Ordering Information
Description Form Package Volume | Size Order Number
4Cell¢ç MDXK Medium Liquid Bottle 1 L | Box of 2 x 1 L CFV3FA2003
4Cell¢ç MDXK Medium Liquid Bottle 1 L | Box of 2 x 1 L CFV3FA2003
4Cell¢ç MDXK Medium Liquid Bottle 1 L | Box of 6 x 1 L CFV3FA2004
4Cell¢ç MDXK Medium Powder Multilayer Bag 10 L CQV3FA2012
4Cell¢ç MDXK Medium Powder Multilayer Bag 100 L CQV3FA2014



 
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