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4Cell® SmartCHOpe for Perfusion Applications
 
- 4Cell® CHO Media and Feed Portfolio (brochure)
- Cell Culture Media and Buffer (brochure)


4Cell® SmartCHOpe media 는 Perfusion Applications 에 사용할 수 있도록 설계된 media 입니다.




Ordering Information
 
4Cell® SmartCHOpe
Media Description Format | Quantity | Package Order Number
4Cell® SmartCHOpe SAM Allows the cultivation or media adaptation of CHO cells Powder | 10 L | Container CQP3FC1101
Powder | 25 L | Container CQP3FC1102
Powder | 250 L | Container CQP3FC1103
4Cell® SmartCHOpe PM Non-selective production medium for perfusion culture Powder | 20 L | Container CQP3FC2102
Powder | 50 L | Container CQP3FC2101
Powder | 200 L | Container CQP3FC2103
4Cell® SmartCHOpe FMA Ideal companion feeds designed to be used together with 4Cell® SmartCHOpe Production Medium Powder | 3 L | Container CQP3FC3103
Powder | 30 L | Container CQP3FC3102
Powder | 120 L | Container CQP3FC3104
4Cell® SmartCHOpe FMB Ideal companion feeds designed to be used together with 4Cell® SmartCHOpe Production Medium Powder | 2 L | Container CQP3FC4104
Powder | 4.5 L | Container CQP3FC4101
Powder | 2 L | Container CQP3FB4154
Powder | 45 L | Container CQP3FC4102
Powder | 180 L | Container CQP3FC410


CHO Media Sample Kits
Product Media Media Format | Quantity | Package Order Number
4Cell® SmartCHOpe
Media System		 Stock & Adaptation Medium (SAM) Powder | 1×10 L | Container CQP3FC0100
Production Medium (PM) Powder | 1×50 L | Container
Feed Medium A (FMA) Powder | 2×3 L | Container
Feed Medium B (FMB) Powder | 1×2 L | Container



Peformance Test


Figure 1 : Performance of 4Cell ® SmartCHOpe in an N-1 Process.
4Cell® SmartCHOpe media 는 N-1 perfusion 공정에 효율적으로 사용됩니다 (Figure 1A).
CHO cell 은 100 million cells/mL 의 고밀도 perfusion cell culture 를 5-7 일동안 진행할 수 있습니다 (Figure 1B).
그 후 200리터 bioreactor 에서 대용량 세포배양을 할 수 있는데 이는 4Cell® SmartCHOpe media 는 standard seed train 에서 탁월한 세포성장과 viability 를 보여주고 있다는 것을 의미합니다 (Figure 1C).
Note. (A) Seed train intensification by N-1 Perfusion, (B) Growth Performance of CHO cells cultured in 4Cell® SmartCHOpe across different seed train scenarios, (C) Growth Performance of CHO cells cultured in 4Cell® SmartCHOpe in a production bioreactor (Biostat® STR 200 L)



Figure 2 : 4Cell® SmartCHOpe Performance in Perfusion Processes
4Cell® SmartCHOpe media 는 연속적인 perfusion applications 을 할 수 있습니다. 오른 쪽 data 는 ATF device 와 연결된 2 Liter Bioreactor 에서 4Cell® SmartCHOpe media 를 이용하여 continuous perfusion 방식으로 세포를 키운 결과를 보여주고 있습니다. 세포는 고밀도, 높은 세포생존율과 생산성을 유지하면서 34일동안 세포배양을 진행할 수 있었다.
Note. Viable cell density (VCD) and percentage viability were measured in a 2 L bioreactor with ATF for 34 days.



Figure 3 : 4Cell ® SmartCHOpe Performance in N-1 Perfusion Followed by High-Inoculation Fed-Batch.
N-1 perfusion 에 의한 high innoculation fed-batch 를 이용하면 CHO clone 에 따라 10 g/L 까지 생산성을 증가시킬 수 있습니다.
Note. N-1 perfusion: cultivation was performed in the Biostat® RM System with 4Cell® SmartCHOpe at 1 L working volume. High-inoculation fed-batch cultivation was carried out in 5 L UniVessel® with 4Cell® SmartCHOpe as basal medium, and 1.3× 4Cell® SmartCHO feeds added to provide adequate nutrient supply throughout the whole process



Figure 4 : 4Cell ® SmartCHOpe Performs Well in a Perfusion Mimic System
4Cell® SmartCHOpe 를 Ambr® 15 perfusion mimic 과 함께 사용하면 perfusion processes 를 위한 적절한 clone 을 evaluation 하는 작업을 효율적으로 할 수 있습니다.
Note. Data from Cellca CHO-DG44 Cell Line Development Service. Four clones cultured in the Ambr® 15.



Technical Specification
 
Media Surfactant Phenol Red L-Glutamine Hypoxanthine/
Thymidine		 Growth
Factor		 Glucose Amino Acids Vitamins
SmartCHO® (SAM|PM) ■ - - - - ■ ■ ■
SmartCHO® FMB - - - - - - ■ -
SmartCHO® FMA ■ - - - - ■ ■ ■
TCXD6 ■ - - ■ ■ ■ ■ ■
TC-42 w/IGF ■ - - - ■ ■ ■ ■
TC-42 w/rInsulin ■ - - - - ■ ■ ■
TC-42 w/o GF ■ - - - - ■ ■ ■
TCX10D ■ - - ■ - ■ ■ ■
CHOlean ■ - - - - ■ ■ ■
CHO TF ■ - - ■ - ■ ■ ■
TCX7D feed ■ - - ■ - 60 g/L ■ ■
Basic Feed ■ - - ■ - 20 g/L ■ ■



 
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