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¿µÁøÄÚÆÛ·¹ÀÌ¼Ç Á¾ÇÕÄ«´Ù·Î±×
A Well-Established Transfection Medium Designed for HEK293 Cell Lines in Suspension Mode
 
- HEK Media and Feed Portfolio (brochure)
- Protocol HEK TF Media (protocol)
- The Journey to AAV Production in Suspension - Scaling-UP Your Process (application note)
- Navigating Upstream AAV Production for Gene Therapy Manufacturing (white paper)


 
 
¡î complete chemically defined, animal-component-free ±×¸®°í and hydrolysate-free medium
¡î HEK ¶Ç´Â ´Ù¸¥ mammalian cell line À» ¹è¾çÇÏ¿© AAV (adeno-associated virus) ¿Í lentivirus ¸¦ transfection ÇÏ°í ÀÌ virus µéÀ» ´ë·®À¸·Î »ý»êÇÏ´Â ¸ñÀûÀ¸·Î °³¹ßµÈ media
¡î AAV ¶Ç´Â lentivirus ¸¦ ÀÌ¿ëÇÏ´Â gene therapy ¿¬±¸ ¹× »ý»ê¿¡ ¸Å¿ì Áß¿äÇÑ media
¡î ÀÌ media ´Â ƯÈ÷ PEI (polyethylenimine) ¿Í °°Àº polycationic transfection reagents ¸¦ »ç¿ëÇÏ´Â transient transfection ¿¡ ¸Å¿ì È¿°úÀûÀÎ media
¡î high-density suspension culture ¿¡¼­ ¼¼Æ÷¸¦ ¹è¾çÇÏ°í recombinant proteins, antibodies ±×¸®°í viral vectors ¸¦ »ý»êÇϴµ¥ »ç¿ëÇÏ´Â media
¡î ´Ù¸¥ serum-free media ¿¡¼­ ¹è¾çÇÑ ¼¼Æ÷µµ adaptation °úÁ¤ ÇÊ¿ä¾øÀÌ ¹è¾ç °¡´ÉÇÑ media
¡î seed train culture ¿¡¼­ suspension cell ÀÇ ¼ºÀåÀ» ¾ÈÁ¤ÀûÀ¸·Î À¯Áö½ÃÄÑÁÖ´Â media
¡î w/ HT (hypoxanthine/thymidine)
¡î w/ growth factor
¡î w/o L-glutamine
¡î arginine °ú lysine ÀÌ ¾ø´Â SILAC variant »ç¿ë °¡´É
¡î DIN EN ISO 9001 ¿Í DIN EN ISO 13485 ¿¡ ÀÇÇØ Quality ½ÂÀÎ



Ordering Information
 
HEK TF Media
Media Description Format | Quantity | Package Order Number
HEK TF Well established transfection medium : Ideal for the production of recombinant proteins and antibodies Liquid | 1 L Bottle 861-0001
Liquid | 10 L Bag 861-0010BAG
Liquid | 20 L Bag 861-0020BAG
Liquid | 50 L Bag 861-0050BAG
Powder & Supplement | 10 L Container CQV3FB4007
Powder & Supplement | 100 L Container CQV3FB4009


HEK Media Sample Kits
Media Description Format | Quantity | Package Order Number
HEK293 Media Sample Kit To select the best fit media for the application and the feed Liquid | 4¡¿1 L Bottle CFV3FB4000



Technical Specifications
 
Attribute Growth
Factor
Hypoxanthine
/ Thymidine
Characteristics
/ Comparison
Production Methods
HEK GM With With ¡á Stable/Robust
¡á High nutrient level
¡á Infection
¡á Stable expression
HEK ViP NX Without With ¡á Highest nutrient level ¡á Transient transfection
¡á Infection
¡á Stable expression
HEK ViP NB Without With ¡á Medium nutrient level
¡á Leanest formulation
  (lowest number of components)
¡á Transient transfection
¡á Infection
¡á Stable expression
HEK TF With With ¡á Broad application ¡á Transient transfection
¡á Infection
¡á Stable expression
HEK FS_2 Feed Without With ¡á Feed Supplement to boost the productivity of the HEK media ¡á Transient transfection (use from 4-6 h post-transfection)
¡á Infection
¡á Stable expression



 
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