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¿µÁøÄÚÆÛ·¹ÀÌ¼Ç Á¾ÇÕÄ«´Ù·Î±×
(curcumin nanoemulsion ¿¡ ´ëÇÑ ½Ä¹°¼º À¯È­Á¦ÀÎ pectin °ú gum Arabic ÀÇ ¾ÈÁ¤¼º È¿°ú ºÐ¼®)
Ilyes Dammak, Paulo Jos¢¥e & Amaral Sobral (2021). Elsevier Ltd., 2212-4292.
|   ¿ø¹®º¸±â   |   ¸®½ºÆ®µ¹¾Æ°¡±â   |

Introduction

pectin °ú gum Arabic À» À¯È­Á¦·Î »ç¿ëÇÏ´Â curcumin (°­È²) nanoemulsion ¿¡¼­ pectin °ú gumArabic À¯È­Á¦°¡ ¿ÜºÎÀÇ ´Ù¾çÇÑ È¯°æ¿¡ ´ëÇØ ¾î´ÀÁ¤µµ stability ¸¦ À¯ÁöÇÏ´ÂÁö È®ÀÎÇϱâ À§ÇØ ½Å¼ÓÇÏ°í Á¤¹ÐÇÑ ºÐ»ê¾ÈÁ¤¼º ºÐ¼®ÀåºñÀÎ LUMiSizer Dispersion Analyzer ¸¦ ÀÌ¿ëÇÏ¿© ¿©·¯°¡Áö Å×½ºÆ®¸¦ ÁøÇàÇÏ¿´´Ù.

Nano-emulsion preparation

Á¤Á¦µÈ soybean oil ¿¡ 0.3% (w/w) curcuminÀ» 12½Ã°£µ¿¾È ºÐ»êÇÑ ÈÄ syringeÇÊÅÍ (0.45 ¥ìm) ¸¦ »ç¿ëÇÏ¿© ºÒ¼ø¹°À» Á¦°ÅÇÑ oil phase ¸¦ Á¦Á¶ÇÏ¿´´Ù. Áõ·ù¼ö¿¡ 0.1~5% (w/w) ´Ù¾çÇÑ ³óµµÀÇ À¯È­Á¦ (gum arabic ¶Ç´Â pectin) ¸¦ ¿ëÇØÇÏ¿© ¿¬¼Ó»ó (continuous phase) À» Á¦Á¶ÇÏ¿´´Ù.

10% (v/v) oil phase ¿Í 90% (v/v) continuous phase ¸¦ È¥ÇÕÇÑ ÈÄ rotor-stator mixier ¸¦ ÀÌ¿ëÇÏ¿© 104 rpm¿¡¼­ 5ºÐ°£ ó¸®ÇÏ¿© premixed emulsionÀ» Á¦Á¶ÇÏ¿´´Ù.

ÀÌ·¸°Ô ÁغñµÈ pre-emulsionÀº Microfluidizer (M-110Y)¿¡¼­ 600 bar, 800 bar, 1000 bar, and 1300 barÀÇ ¾Ð·ÂÇÏ¿¡¼­ ÃÑ 4 pass¸¦ °ÅÃÄ curcumin nano-emulsion·Î ¿Ï¼ºµÇ¾ú´Ù.

Dispersion Properties of Nanoemulsion

°¡Àå ÀÛÀº oil droplet Å©±â¸¦ º¸¿©ÁØ 3% (w/w) gum arabic ¿Í 2% (w/w) pectin µÎ°¡Áö »ùÇà Áß ¾î´À »ùÇÃÀÇ ¾ÈÁ¤¼ºÀÌ ´õ ÁÁÀºÁö È®ÀÎÇϱâ À§ÇØ ÀüÅëÀûÀÎ ÀÚ¿¬Á߷¹æ½ÄÀÇ ºÐ»ê¾ÈÁ¤¼º ºÐ¼®Àåºñº¸´Ù ÈξÀ ´õ Á¤¹ÐÇÏ°í ½Å¼ÓÇÑ °á°ú¸¦ ¾Ë ¼ö ÀÖ´Â LUMiSizer Dispersion Analyzer¸¦ ÀÌ¿ëÇÏ¿© ÀÌ µÎ »ùÇÃÀÇ ºÐ»ê¾ÈÁ¤¼ºÀ» ºÐ¼®ÇÏ¿´°í [±×¸² (a), (b)], ¶ÇÇÑ laser confocal microscopy ¸¦ ÀÌ¿ëÇÏ¿© µÎ nanoemulsion »ùÇÃÀÇ droplet microstructure ¸¦ ÃÔ¿µÇÏ¿´´Ù [±×¸² (c)].



Fig. 3. Transmission profiles of curcumin-loaded nanoemulsions stabilized with (a) 3% gum Arabic, and (b) 2% pectin; and (c) microstructure of nanoemulsion droplets after oil labelling with Nile Red, insert bar is one ¥ìm. All nanoemulsions were produced with homogenization pressure of 100 MPa, 4 passes. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article


±×¸²°ú °°ÀÌ (a) gum arabic¸¦ À¯È­Á¦·Î ÇÑ nanoemulsion»ùÇÃÀÇ transmission profile Àº ½ÃÁ¾ ¿Ï¸¸ÇÑ ±â¿ï±â·Î ÀÌ·ç¾î Áø ±×·¡ÇÁ°¡ »ý¼ºµÇ¾ú´Âµ¥ ÀÌ´Â ÀÌ (a) gum arabic À¯È­Á¦°¡ µé¾î°£ nanoemulsion »ùÇÃÀº ¸Å¿ì ¾ÈÁ¤ÀûÀ̶ó´Â °ÍÀ» ÀǹÌÇÑ´Ù.

¹Ý¸é (b) pectinÀ» À¯È­Á¦·Î »ç¿ëÇÑ nanoemulsion»ùÇÃÀÇ transmission profile Àº ¸Å¿ì ºÒ±ÔÄ¢ÀûÀÎ Áøµ¿À» º¸ÀÌ°í ÀÖ´Ù. ÀÌ·¯ÇÑ ÇüÅÂÀÇ transmission profile Àº ÀÌ »ùÇà ³»ºÎ¿¡¼­ droplet ÀÌ ¼­·Î aggregation ÇÏ¿© droplet ÀÌ Á¸ÀçÇÑ´Ù´Â °ÍÀ» ÀǹÌÇÏ¸ç °á±¹ ÀÌ nanoemulsion»ùÇÃÀº ¾ÈÁ¤¼ºÀÌ ¸Å¿ì ¶³¾îÁø´Ù´Â °ÍÀ» ÀǹÌÇÑ´Ù.

laser confocal microscopy·Î È®ÀÎÇÑ µÎ nanoemulsion»ùÇà droplet ÀÇ microstrucure ¿ª½Ã [±×¸² (c)] gum arabic À¯È­Á¦¸¦ »ç¿ëÇÑ nanoemulsion ÀÇ droplet Å©±â´Â ¸Å¿ì ÀÏÁ¤Çѵ¥ ¹ÝÇØ pectin À» À¯È­Á¦·Î »ç¿ëÇÑ nanoemulsion ÀÇ droplet Àº aggregation ÀÌ ÀϾ droplet Å©±â°¡ ¸Å¿ì ´Ù¾çÇÏ°Ô ±¸¼ºµÇ¾î ÀÖ´Ù´Â °ÍÀ» ¾Ë ¼ö ÀÖ¾ú´Ù. ÀÌ°ÍÀº ±×¸² (a) ¿Í (b) ÀÇ °á°ú¿Í ÀÏÄ¡ÇÏ¿´´Ù.

Effect of other conditions

À§¿Í °°Àº ºÐ¼® ¹æ¹ýÀ¸·Î temperature, pH, salt concentration, freeze-thaw treatment ¿Í °°Àº ´Ù¾çÇÑ Á¶°Ç¿¡¼­ pectin °ú gum Arabic À» À¯È­Á¦·Î »ç¿ëÇÏ´Â curcumin (°­È²) nanoemulsion ÀÇ ¾ÈÁ¤¼ºÀ» ºÐ¼®ÇÏ¿´´Ù.

Conclusions

¹Ì±¹ Microfluidics »çÀÇ Microfluidizer °í¾ÐºÐ»êÀåºñ¸¦ ÀÌ¿ëÇÏ¿© ½ÄÇ®¼º õ¿¬ À¯È­Á¦ÀÎ pectin ¶Ç´Â gum Arabic À» À¯È­Á¦·Î ÷°¡µÈ Curcumin Nanoemulsion (°­È²) À» ¼º°øÀûÀ¸·Î Á¦Á¶ÇÏ¿´°í µ¶ÀÏ LUM »çÀÇ ºÐ»ê¾ÈÁ¤¼º ºÐ¼®ÀåºñÀÎ LUMiSizer Dispersion Analyser ¸¦ ÀÌ¿ëÇÏ¿© ´Ù¾çÇÑ Á¶°Ç¿¡¼­ ¾î¶² À¯È­Á¦°¡ Curcumin Nanoemulsion À» ´õ ¾ÈÁ¤ÇÏ°Ô À¯Áö½ÃÅ°´ÂÁö È®ÀÎÇÏ¿´´Ù.




 
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