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¿µÁøÄÚÆÛ·¹ÀÌ¼Ç Á¾ÇÕÄ«´Ù·Î±× |
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iQue¢ç Mouse T Cell Kit ´Â T ¼¼Æ÷ÀÇ activation, memory, ±×¸®°í exhaustion À» ÃøÁ¤ÇÏ°í µ¿½Ã¿¡ cytokine ºÐºñ¿¡ ´ëÇÑ ÀÌ ¼¼Æ÷µéÀÇ ¿ªÇÒ, ±×¸®°í °¢ ¼¼Æ÷ÀÇ Health °ü·Ã Á¤º¸¸¦ Á¦°øÇÕ´Ï´Ù.
ÀÌ Kit ¸¦ »ç¿ëÇÏ¸é ´ÙÀ½°ú °°Àº ÀåÁ¡ÀÌ ÀÖ½À´Ï´Ù. |
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fluorescent membrane integrity dye ¸¦ ÀÌ¿ëÇÏ¿© »ì¾ÆÀÖ´Â ¸é¿ª¼¼Æ÷¸¸ ÃøÁ¤ÇÕ´Ï´Ù. |
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¼¼Æ÷µéÀ» fluorescent antibody panel ·Î ¿°»öÇÏ¿© CD3+ T cells, CD3- non-T cells, CD4+ T helper cells, CD8+ T cytotoxic cells µîÀ¸·Î ºÐ¸®ÇÏ¿© ¼¼Æ÷¸¦ phenotyping ÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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mouse T cell activation, memory ±×¸®°í exhaustion ¿¡ ´ëÇÑ 4°¡Áö ´Ù¸¥ Á¾·ùÀÇ T cell surface marker ¸¦ Æ÷ÇÔÇÏ°í ÀÖ½À´Ï´Ù. |
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ÃÖÀûÈµÈ ½ÇÇèÈ帧¿¡ ÀÇÇØ activated T cell ¿¡ ÀÇÇØ ºÐºñµÈ pro-inflammatory cytokine IFN¥ã and anti-inflammatory cytokine IL-10 °ú °°Àº effector cytokine À» ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù |
Key Benefits | |
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Speed : ¿©·¯ Á¾·ùÀÇ assay ¸¦ µ¿ÀÏÇÑ well ¿¡¼ µ¿½Ã¿¡ ÁøÇà°¡´ÉÇÏ¿© ½Ã°£, ºñ¿ë ±×¸®°í ¼ÒÁßÇÑ ½Ã·áÀÇ »ç¿ëÀ» ÁÙÀÏ ¼ö ÀÖ½À´Ï´Ù. |
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Results : ÇϳªÀÇ assay well ¿¡¼ mouse ÀÇ T cell activation, memory, exhaustion, cell viability, cell proliferation, and secreted pro/anti-inflammatory cytokines ¸¦
µ¿½Ã¿¡ ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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Biological insights : ÇϳªÀÇ plate well ¿¡ ÀÖ´Â ¼¼Æ÷¿Í cytokine À» µ¿½Ã¿¡ È®ÀÎÇÒ ¼ö ÀÖÀ¸¹Ç·Î µ¿ÀÏÇÑ ¼¼Æ÷ÀÇ Health ¿Í Function °ü·Ã µ¥ÀÌÅ͸¦ ±¸ÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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Simplicity : ÇϳªÀÇ platform ¿¡¼ ½ÇÇè°ú µ¥ÀÌÅÍ ºÐ¼®ÀÌ µ¿½Ã¿¡ ÁøÇàÇÒ ¼ö ÀÖÀ¸¹Ç·Î ½Ã°£À» ´ëÆø ÁÙÀÏ ¼ö ÀÖ½À´Ï´Ù. |
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ÇϳªÀÇ well ¿¡¼ cell viability, activation °ú exhaustion °ü·Ã T cell phenotypes markers, secreted cytokines, cell encoding °ú proliferation (optional) À» µ¿½Ã¿¡ ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù.
T cell proliferation or encoded target cells ÃøÁ¤µµ °¡´ÉÇϳª ÀÌ ¼³¸í¿¡´Â »ý·«µÇ¾î ÀÖ½À´Ï´Ù. |
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Workflow of iQue¢ç Mouse T Cell Kit |
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