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¿µÁøÄÚÆÛ·¹ÀÌ¼Ç Á¾ÇÕÄ«´Ù·Î±× |
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iQue¢ç Human T Cell Exhaustion Kit ´Â T ¼¼Æ÷ÀÇ exhaustion ¿¡ ´ëÇÑ ´Ù¾çÇÑ marker ¸¦ ¸ÖƼÇ÷º½ÌÇÏ¿© »ç¿ëÇϱ⠽±µµ·Ï ¼³°èµÇ¾ú½À´Ï´Ù.
¶ÇÇÑ T helper and T cytotoxic cells ÀÇ phenotyping, cell health, secreted cytokines ÀÇ bead-based ÃøÁ¤ µîÀ» ÇѹøÀÇ assay ¿¡¼ µ¿½Ã¿¡ ÁøÇà °¡´ÉÇÕ´Ï´Ù.
ÀÌ Kit ¸¦ »ç¿ëÇÏ¸é ´ÙÀ½°ú °°Àº ÀåÁ¡ÀÌ ÀÖ½À´Ï´Ù. |
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fluorescent membrane integrity dye ¸¦ ÀÌ¿ëÇÏ¿© »ì¾ÆÀÖ´Â ¸é¿ª¼¼Æ÷¸¸ ÃøÁ¤ÇÕ´Ï´Ù. |
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¼¼Æ÷µéÀ» fluorescent antibody panel ·Î ¿°»öÇÏ¿© CD3+ T cells, CD3- non-T cells, CD3+CD4+ helper T cells, and CD3+CD8+ cytotoxic T cell µîÀ¸·Î ºÐ¸®ÇÏ¿© ¼¼Æ÷¸¦ phenotyping ÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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3°¡Áö Á¾·ùÀÇ T cell exhaustion marker (PD-1, Tim-3, and Lag-3) ¸¦ Æ÷ÇÔÇÏ°í ÀÖ½À´Ï´Ù. |
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ÃÖÀûÈµÈ ½ÇÇèÈ帧¿¡ ÀÇÇØ activated T cell ¿¡ ÀÇÇØ ºÐºñµÈ IFN¥ã ¿Í TNF °°Àº cytokine À» ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù. |
Key Benefits | |
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Speed : ¿©·¯ Á¾·ùÀÇ assay ¸¦ µ¿ÀÏÇÑ well ¿¡¼ µ¿½Ã¿¡ ÁøÇà°¡´ÉÇÏ¿© ½Ã°£, ºñ¿ë ±×¸®°í ¼ÒÁßÇÑ ½Ã·áÀÇ »ç¿ëÀ» ÁÙÀÏ ¼ö ÀÖ½À´Ï´Ù. |
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Results : ÇϳªÀÇ assay well ¿¡¼ °¢±â ´Ù¸¥ stage ¿¡¼ÀÇ T cell memory phenotypes °ú function, ±×¸®°í cell viability, cell count, secreted pro/anti-inflammatory cytokines ¸¦
µ¿½Ã¿¡ ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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Biological insights : ÇϳªÀÇ plate well ¿¡ ÀÖ´Â ¼¼Æ÷¿Í cytokine À» µ¿½Ã¿¡ È®ÀÎÇÒ ¼ö ÀÖÀ¸¹Ç·Î µ¿ÀÏÇÑ ¼¼Æ÷ÀÇ Health ¿Í Function °ü·Ã µ¥ÀÌÅ͸¦ ±¸ÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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Simplicity : ÇϳªÀÇ platform ¿¡¼ ½ÇÇè°ú µ¥ÀÌÅÍ ºÐ¼®ÀÌ µ¿½Ã¿¡ ÁøÇàÇÒ ¼ö ÀÖÀ¸¹Ç·Î ½Ã°£À» ´ëÆø ÁÙÀÏ ¼ö ÀÖ½À´Ï´Ù. |
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ÇϳªÀÇ plate well ¿¡¼ cell viability, T cell phenotypes, T cell exhaustion markers, secreted cytokines, cell encoding and proliferation (optional) À» µ¿½Ã¿¡ ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù.
T cell proliferation or encoded target cells ÃøÁ¤µµ °¡´ÉÇϳª ÀÌ ¼³¸í¿¡´Â »ý·«µÇ¾î ÀÖ½À´Ï´Ù. |
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Workflow of iQue¢ç Human T Cell Exhaustion Kit |
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