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¿µÁøÄÚÆÛ·¹ÀÌ¼Ç Á¾ÇÕÄ«´Ù·Î±×
¡ì Immune Cell Phenotyping and Functions µ¹¾Æ°¡±â


iQue¢ç Human T Cell Activation Kit ´Â ÀϹÝÀûÀÎ ½ÇÇè°úÁ¤À» °£¼ÒÈ­ÇÏ¿© ¸é¿ª ¼¼Æ÷ phenotype, T cell activation ÀÇ ÃÊ±â ¹× Èıâ Ç¥ÁöÀÚ, cell proliferation ¶Ç´Â encoded target cells, cell viability ±×¸®°í ºÐºñµÈ cytokine ³óµµ¸¦ ´Ü 5 - 10ul ÀÇ ¼Ò·®ÀÇ ½Ã·á·Î ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù
iQue¢ç Human T Cell Activation and Cytokine Profiling Kit ´Â T ¼¼Æ÷ÀÇ È°¼ºÈ­ Á¤µµ¿¡ ´ëÇÑ Á¤º¸¸¦ Á¦°øÇϸ鼭 µ¿½Ã¿¡ ¼¼Æ÷ÀÇ Health »óÅÂ¿Í cytokine ºÐºñ Á¤µµ¿¡ ´ëÇÑ Á¤º¸µµ Á¦°øÇÕ´Ï´Ù. one washing ¸¸ ÇϹǷΠ½ÇÇè ÁøÇàÀÌ ¸Å¿ì °£ÆíÇÏ°í ¼¼Æ÷¿Í ºñµå¸¦ µ¿½Ã¿¡ ÃøÁ¤ÇϹǷΠ´ÙÀ½°ú °°Àº »ý¹°ÇÐÀû ÀÌÇظ¦ Çϴµ¥ ¸¹Àº µµ¿òÀ» Á¦°øÇÕ´Ï´Ù.
fluorescent membrane integrity dye ¸¦ ÀÌ¿ëÇÏ¿© »ì¾ÆÀÖ´Â ¸é¿ª¼¼Æ÷¸¸ ÃøÁ¤ÇÕ´Ï´Ù.
¼¼Æ÷µéÀ» fluorescent antibody panel ·Î ¿°»öÇÏ¿© CD3+ T cells, CD3- non-T cells, CD4+ T helper cells, CD8+ T cytotoxic cells µîÀ¸·Î ºÐ¸®ÇÏ¿© ¼¼Æ÷¸¦ phenotyping ÇÒ ¼ö ÀÖ½À´Ï´Ù.
T cell phenotypes ´Â CD69 (early), CD25 (late), and HLA-DR (even later, with strong stimulation) °ú °°Àº 3 °¡Áö È°¼º Ç¥ÁöÀÚ¿¡ ´ëÇØ ÇÁ·ÎÆÄÀϸµ µÇ¾îÁý´Ï´Ù.
ÃÖÀûÈ­µÈ ½ÇÇèÈ帧¿¡ ÀÇÇØ activated T cell ¿¡ ÀÇÇØ ºÐºñµÈ Th1 cytokine IFN¥ã/ multifunctional cytokine TNF¥á °ú °°Àº effector cytokine À» ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù.


Key Benefits
¡î Speed : ¿©·¯ Á¾·ùÀÇ assay ¸¦ µ¿ÀÏÇÑ well ¿¡¼­ µ¿½Ã¿¡ ÁøÇà°¡´ÉÇÏ¿© ½Ã°£, ºñ¿ë ±×¸®°í ¼ÒÁßÇÑ ½Ã·áÀÇ »ç¿ëÀ» ÁÙÀÏ ¼ö ÀÖ½À´Ï´Ù.
¡î Results : ÇϳªÀÇ assay well ¿¡¼­ T cell phenotypes, °¢ ÇüÁú¿¡¼­ÀÇ early and late cell activation markers, cell viability, cell count, proliferation and secreted effector cytokines ¸¦ µ¿½Ã¿¡ ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù.
¡î Biological insights : ÇϳªÀÇ plate well ¿¡ ÀÖ´Â ¼¼Æ÷¿Í cytokine À» µ¿½Ã¿¡ È®ÀÎÇÒ ¼ö ÀÖÀ¸¹Ç·Î µ¿ÀÏÇÑ ¼¼Æ÷ÀÇ Health ¿Í Function °ü·Ã µ¥ÀÌÅ͸¦ ±¸ÇÒ ¼ö ÀÖ½À´Ï´Ù.
¡î Simplicity : ÇϳªÀÇ platform ¿¡¼­ ½ÇÇè°ú µ¥ÀÌÅÍ ºÐ¼®ÀÌ µ¿½Ã¿¡ ÁøÇàÇÒ ¼ö ÀÖÀ¸¹Ç·Î ½Ã°£À» ´ëÆ÷ ÁÙÀÏ ¼ö ÀÖ½À´Ï´Ù.
¡î Automation friendly : ¾ÆÁÖ ¸¹Àº ¼öÀÇ ½Ã·áÀÇ screening À» ÀÚµ¿À¸·Î ÁøÇàÇÒ ¼ö ÀÖ¾î Ź¿ùÇÑ °á°ú ÀçÇö¼ºÀ» °¡Áø µ¥ÀÌÅ͸¦ º¼ ¼ö ÀÖ½À´Ï´Ù.



ÇϳªÀÇ well ¿¡¼­ T cell phenotypes, activation markers, cell proliferation, cell viability, ±×¸®°í ºÐºñµÈ cytokines µéÀ» µ¿½Ã¿¡ ÃøÁ¤ÀÌ ÀÌ·ç¾î Áý´Ï´Ù. ±×¸®°í ÀÌ µµÇü¿¡´Â ºüÁ® ÀÖÀ¸³ª T cell proliferation ¶Ç´Â encoded target cells ÀÇ ÃøÁ¤µµ °¡´ÉÇÕ´Ï´Ù.



The Workflow of iQue¢ç Human T Cell Activation Kit



 
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