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¿µÁøÄÚÆÛ·¹ÀÌ¼Ç Á¾ÇÕÄ«´Ù·Î±× |
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iQue¢ç Human and Mouse Antibody Internalization Reagents |
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´ë±Ô¸ð Ç×ü±ºÀ» ½Å¼ÓÇÏ°Ô Æò°¡, ºñ±³ÇÏ°í antibody internalization °ú °°Àº Ç×üÀÇ ÇÙ½É Æ¯¼ºÀ» È®ÀÎÇÏ¿© »õ·Î¿î Èĺ¸ ¼±Á¤¿¡ ÇÊ¿äÇÑ ½Ã°£À» Å©°Ô ´ÜÃà½ÃÅ°°í
°¡´É¼ºÀÖ´Â Ä¡·á¹ýÀÇ °³¹ßÀ» ºü¸£°Ô ÁøÇàÇÒ ¼ö ÀÖ½À´Ï´Ù.
iQue¢ç Human and Mouse Antibody Internalization Reagents ´Â washing ÀÌ ÇÊ¿ä¾ø´Â pH sensitive Dye ·Î¼ plate ¿¡¼ 20 §¡ sample À» ÀÌ¿ëÇÏ¿© antibody internalization À» È®ÀÎÇϴµ¥ »ç¿ëµË´Ï´Ù.
ÀÌ assay ´Â ´Ù¸¥ °ËÁõµÈ ½Ã¾àÀ» ÀÌ¿ëÇÏ¿© µ¿½Ã¿¡ ¿©·¯°³ÀÇ ½ÇÇèÀ» ´ë¿ë·®À¸·Î ÁøÇàÇÒ ¼ö Àִ Ư¼ºÀ» °¡Áö°í ÀÖ½À´Ï´Ù. |
iQue¢ç Human and Mouse Antibody Internalization Kits |
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iQue¢ç Human and Mouse Antibody Internalization Kits ´Â ÇϳªÀÇ 30 §¡ assay volume À¸·Î ºÎÅÍ antibody internalization, antibody specificity, ±×¸®°í cell health ¸¦ µ¿½Ã¿¡
´ë¿ë·®À¸·Î ÃøÁ¤ÇÒ ¼ö ÀÖ´Â no-wash assay kit ÀÔ´Ï´Ù.
Ç×ü´Â pH-sensitive iQue¢ç Antibody Internalization Reagent (R/Red) ·Î ½±°Ô labeling ÇÒ ¼ö ÀÖÀ¸¸ç
¿©±â¿¡ iQue¢ç Cell Membrane Integrity Dye (B/Green) ¸¦ ³Ö°Ô µÇ¸é cell health ¿Í antibody function À» µ¿½Ã¿¡ ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù.
¶ÇÇÑ ÀÌ sample ¿¡ iQue¢ç Cell Proliferation and Encoding Dye V/Blue (Tag-it Violet¢â) ¸¦ ³Ö°Ô µÇ¸é cell specificity µµ µ¿½Ã¿¡ ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù.
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Assay principle of the iQue¢ç Human and Mouse Antibody Internalization Reagents and Kits :: Antibody Internalization Reagent ·Î labeling µÈ Ç×ü´Â
Áß¼º pH ¿¡¼ Çü±¤À» »ý¼ºÇÏÁö ¾ÊÀ¸³ª Ç×ü°¡ ¼¼Æ÷ ¾ÈÀ¸·Î internalization µÇ¾îÁö°í »ê¼ºÈ¯°æÀÎ lysosome °ú endosome À¸·Î µé¾î°¡°Ô µÇ¸é °ÇÑ Çü±¤ÀÌ ¹ß»ýÇÏ°Ô µÈ´Ù. |
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(A) Pre-set template gates are provided for different populations (cells, single cells, and internalized antibodies).
The template also provides a dose response curve plotted for MFI and percent internalized for a control sample.
Additional curves for further samples can be generated, including (B), the number of cells with internalized antibodies as a percentage of single cells. |
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