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¿µÁøÄÚÆÛ·¹ÀÌ¼Ç Á¾ÇÕÄ«´Ù·Î±× |
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ISO 15598:1999 Tea - Determination of crude fibre content
Tested with VELP Scientifica FIWE 3 Fiber Analyzer (Code F30520201) |
Introduction
¼¶À¯ÁúÀº »ç¶÷ÀÇ À§Àå°ü¿¡¼ ¼ÒÈ ¹× Èí¼öµÉ ¼ö ¾ø´Â °úÀÏ ¹× ä¼ÒÀÇ lignin, cellulose, hemicellulose, pectinÀ» ¸»ÇÑ´Ù.
¼¶À¯ ºÐ¼®Àº ´ÙÀ½°ú °°Àº °æ¿ì¿¡ Áß¿äÇÏ´Ù.
- ¿µ¾çÀû ÀÌÀ¯: ¼¶À¯ÁúÀÇ ¾çÀº ¼Òȱâ°üÀÇ °Ç°°ú ±â´É¿¡ Áß¿äÇÑ ÀÛ¿ëÀ» ÇÑ´Ù. ¼¶À¯ÁúÀÌ ³Ê¹« ¸¹À¸¸é low fiber intake irregularity ¼ÒÈ ±â°ü Àå¾Ö°¡ ¹ß»ýÇÒ ¼ö ÀÖ´Ù.
- ¹ýÀû ÀÌÀ¯: °ÅÀÇ ¸ðµç ±¹°¡ÀÇ ´ç±¹Àº ½ÄÇ° ¹× »ç·á Á¦Ç° Á¦Á¶¾÷ü°¡ ¿µ¾ç Ç¥ÀÇ ÀϺηΠ¼¶À¯Áú ÇÔ·®À» ±âÀÔÇϵµ·Ï ¿ä±¸ÇÑ´Ù.
ÈÇÐÀûÀ¸·Î ¼¶À¯ÁúÀº Èñ¼®µÈ alkali¿Í acid ¿ë¾×À¸·Î ²úÀÎ ÈÄ ºÐÇصÇÁö ¾Ê´Â ÀÜ·ù¹°·Î Á¤ÀǵȴÙ.
Fiber Determination in Tea according to the ISO 15598:1999
ÀûÀýÇÏ°Ô ºÐ¼âµÈ ½Ã·á¸¦ ²ú´Â Ȳ»ê ¿ë¾×°ú ¼ö»êȳªÆ®·ý ¿ë¾×À¸·Î ó¸®ÇÑ´Ù.
¿©°ú °úÁ¤À» ÅëÇØ ÀÜ·ù¹°À» ºÐ¸®ÇÑ ÈÄ ¼¼Ã´ ¹× °ÇÁ¶ÇÏ¿© ¹«°Ô¸¦ ÃøÁ¤ÇÏ°í muffle¿¡¼ °í¿ÂÀ¸·Î °¡¿ÇÑ´Ù.
°í¿Â °¡¿·Î ÀÎÇÑ Áú·® ¼Õ½ÇÀÌ Á¶¼¶À¯ ÇÔ·®ÀÌ´Ù.
Reagents
- Celite 545
- H2SO4 0.255 M: 125 ml H2SO4 20 % in 2 L
- NaOH 0.313 M: 1.5 g NaOH in pellets dissolved in 1 L
- Octanol
- HCl 1 %
- Ethanol > 95 %
- Acetone
Analysis Procedure
¾Æ·¡ Ç¥´Â ºÐ¼® °úÁ¤À» º¸¿©ÁØ´Ù.
MillÀ» »ç¿ëÇÏ¿© »ùÇÃÀ» ÀÔÀÚ Å©±â 1mm·Î ºÐ¼âÇÑ´Ù.
1 gÀÇ celite¿Í 1 gÀÇ °í¸£°Ô ºÐ¼âµÈ »ùÇà (W1)À» ¡¾1mgÀÇ Á¤È®µµ·Î crucible (P1 type - Code A0000139)¿¡ ´ãÀº ÈÄ FIWE ÀåÄ¡¿¡ ¹èÄ¡ÇÑ´Ù.
¿¹¿µÈ 150mlÀÇ 0.255 M H2SO4¿ë¾×¿¡ octanol 3-4¹æ¿ïÀ» Ãß°¡ÇÑ´Ù. ¿ë¾×ÀÌ ²ú±â ½ÃÀÛÇؼ ºÎÅÍ Á¤È®È÷ 30ºÐ µ¿¾È ´õ ²úÀδÙ. ¸¶Áö¸·¿¡ Áø°øÆßÇÁ·Î »ê¼º ¿ë¾×À» ¹èÃâÇÑ´Ù.
Water spray device (A00001135)¸¦ »ç¿ëÇÏ¿© glass column¿¡ ³²¾Æ ÀÖÀ» ¼ö ÀÖ´Â ½Ã·á ÀÜ·ù¹°À» Á¦°ÅÇÑ´Ù. µÎ ¹ø° ½Ã¾àÀ¸·Î 0.313M NaOH ¿ë¾×À» »ç¿ëÇÏ¿© ¾Õ¿¡¼ ÁøÇàÇÑ ÀÛ¾÷À» ¹Ýº¹ÇÑ´Ù.
¿°»ê ¿ë¾×°ú ²ú´Â ¹°À» »ç¿ëÇÏ¿© ÀÜ·ù¹°À» Çѹø ´õ Ç󱺴Ù.
±×·± ´ÙÀ½ >95% ethanol·Î 2ȸ, acetoneÀ¸·Î 3ȸ Ç󱺴Ù.
¹°±â¸¦ »©°í crucibleÀ» ¿Àºì 103¡É¿¡¼ 2½Ã°£ µ¿¾È °ÇÁ¶ÇÑ ÈÄ °ÇÁ¶ Áß·®À» ÃøÁ¤ÇÑ´Ù.
This weight represents the crucible containing crude fiber and ashes (W2).
Place the crucibles in a muffle at 550C, starting from room temperature, for three hours and weigh again after cooling in a desiccator. This weight represents the crucible containing ashes (W3).
¹«°Ô°¡ ÀÏÁ¤ÇÏ°Ô µÉ ¶§±îÁö crucibleÀ» desiccator¿¡¼ ³Ã°¢ÇÑ´Ù.
ÀÌ ¹«°Ô´Â Á¶ ¼¶À¯¿Í ash (W2)°¡ Æ÷ÇÔµÊ crucibleÀÇ ¹«°Ô¸¦ ³ªÅ¸³½´Ù.
Crucible¸¦ 550¡É muffle¿¡ ³Ö°í 3½Ã°£ µ¿¾È °¡¿ÇÑ ÈÄ desiccator¿¡¼ ³Ã°¢ÇÏ¿© ¹«°Ô¸¦ ÃøÁ¤ÇÑ´Ù. ÀÌ ¹«°Ô´Â ash (W3)¸¦ Æ÷ÇÔÇÏ´Â crucibleÀÇ ¹«°Ô¸¦ ³ªÅ¸³½´Ù.
Calculation
% Fiber = ((W2 ? W3) / W1 ) * 100
W1 = sample weight (1g)
W2 = crucible weight with fiber and ashes, after drying in an oven at 103 ¡ÆC for 2 h W3 = crucible weight with ashes, after muffle at 550 ¡ÆC for three hours
Typical Results on Classic Tea (repeated twice)
Á¶ ¼¶À¯ ÇÔ·®Àº °ÇÁ¶µÈ »ùÇà ¹«°Ô ´ëºñ ¹éºÐÀ² (%)·Î Ç¥½ÃµÈ´Ù
Conclusion
½Å·Ú¼º ³ôÀº ºÐ¼®±âÀÎ FIWE ÀåÄ¡¸¦ »ç¿ëÇÏ¸é ºÐ¼® Á¶°ÇÀ» ¾ÆÁÖ ½±°Ô Ç¥ÁØÈÇÒ ¼ö ÀÖ´Ù.
FIWE ½Ã¸®Áî´Â teaÀÇ ¼¶À¯Áú ÃøÁ¤¿¡ ÀûÇÕÇÑ ºÐ¼®±âÀÌ´Ù.
Benefits of FIWE
¨ç FIWE 3 ºÐ¼®±â´Â µ¿½Ã¿¡ 3°³ »ùÇà FIWE 6 ºÐ¼®±â´Â µ¿½Ã¿¡ 6°³ »ùÇÃÀ» ºÐ¼®ÀÌ °¡´ÉÇÏ´Ù
¨è ¼öµ¿ ºÐ¼®¿¡ ºñÇØ ¾à 3¹èÀÇ ½Ã°£À» Àý¾àÇÒ ¼ö ÀÖ´Ù
¨é Filtration, pump, air pressure ÀÏüÇüÀ¸·Î »ç¿ëÀÌ Æí¸®ÇÏ´Ù
¨ê RSD ¡¾1% ÀÌ»óÀÇ Á¤¹Ðµµ·Î ³ôÀº ÀçÇö¼ºÀ» º¸¿©ÁØ´Ù
¨ë Á¤¹Ðµµ: °á°úÀÇ ³ôÀº ÀçÇö¼º: »ó´ë ¡¾1% ÀÌ»ó
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