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¿µÁøÄÚÆÛ·¹ÀÌ¼Ç Á¾ÇÕÄ«´Ù·Î±× |
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iQue¢ç Human T Cell Phenotyping Kit (CD3, CD4 and CD8) ´Â human T cell ÀÇ Subset À» È®ÀÎÇϴµ¥ »ç¿ëÇÏ´Â Kit ÀÔ´Ï´Ù.
ÀÌ Assay Kit ´Â iQue¢ç 3 VBR and VYB configurations ¿¡¼ »ç¿ëÇϵµ·Ï ÃÖÀûȵǾî ÀÖ¾î ¾ÆÁÖ ¸¹Àº ¼öÀÇ sample À» »ç¿ëÇÒ ¼ö ÀÖ½À´Ï´Ù.
ºñƯÀÌÀûÀÎ background staining À» ÃÖ¼ÒȽÃÄ×À¸¸ç cytokine À» Ãß°¡·Î »ç¿ëÇÏ¿© subpopulation Ư¼ºµµ ºÐ¼®ÇÒ ¼ö ÀÖµµ·Ï ±¸¼ºµÇ¾î ÀÖ½À´Ï´Ù. |
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fluorescent antibody panel À» CD3+, CD4+ ±×¸®°í CD8+ ¼¼Æ÷¸¦ ºÐ¸®ÇÏ¿© T ¼¼Æ÷¸¦ phenotyping ÇÕ´Ï´Ù. |
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Qbead Panel À» ÀÌ¿ëÇϸé cytpkine È®Àΰú ¼¼Æ÷ phnotyping À» µ¿½Ã¿¡ ÁøÇàÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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markers ¿Í cytokines À» Ãß°¡·Î »ç¿ëÇÏ¿© T ¼¼Æ÷ subpopulation ÀÇ Æ¯¼ºÀ» ºÐ¼®ÇÏ´Â ÀÛ¾÷µµ ÁøÇàÀÌ °¡´ÉÇÕ´Ï´Ù. |
Key Benefits | |
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High-throughput : ¼Ò·®ÀÇ ¼¼Æ÷ »ùÇÃÀ» [10ul (96-well format) and 5ul (384-well format)] ÀÌ¿ëÇÏ¿© ´ë·®ÀÇ T ¼¼Æ÷¸¦ µ¿½Ã¿¡ immunophenotyping ÀÌ °¡´ÉÇÕ´Ï´Ù. |
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Biological insights : ÇϳªÀÇ plate well ¿¡ ÀÖ´Â ¼¼Æ÷ÀÇ phenotype °ú cell health ¸¦ µ¿½Ã¿¡ ºÐ¼®ÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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Flexibility : markers ¿Í cytokines À» Ãß°¡·Î »ç¿ëÇÏ¿© T ¼¼Æ÷ subpopulation ÀÇ Æ¯¼ºÀ» ºÐ¼®ÇÏ´Â ÀÛ¾÷µµ ÁøÇàÀÌ °¡´ÉÇÕ´Ï´Ù |
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CD3|CD28 Ç×ü¸¦ ÀÌ¿ëÇÏ¿© 3Àϵ¿¾È È°¼ºÈ½ÃŲ human PBMC ÀÇ Phenotyping ºÐ¼®ÀÔ´Ï´Ù.
(A) debris | dead ¼¼Æ÷¸¦ Á¦°ÅÇÏ°í Live Cell ¸¸À¸·Î µÈ Gate ¸¦ ¼³Á¤ÇÕ´Ï´Ù.
(B) ÀÌÁßÀ¸·Î °ãÃÄÁø Á¦°ÅÇÏ°í »ì¾ÆÀÖ´Â ÇϳªÀÇ ¼¼Æ÷µé¸¸À» ¼±Á¤ÇÕ´Ï´Ù.
(C) ÇϳªÀÇ ¼¼Æ÷¸¸À¸·Î µÈ CD3+ cell ÀÔ´Ï´Ù.
(D) CD3+ ¼¼Æ÷ÀÇ ÇÏÀ§Áý´ÜÀÎ CD4+ ¿Í CD8+ ÀÔ´Ï´Ù. |
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Workflow of iQue¢ç Human T Cell Phenotyping Kit (CD3, CD4 and CD8) |
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