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¿µÁøÄÚÆÛ·¹ÀÌ¼Ç Á¾ÇÕÄ«´Ù·Î±× |
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iQue¢ç Human NK Cell Killing Kit ´Â Cell and Bead Mixture Assay Kit ·Î¼ cell phenotype markers, NK cell functional markers,
target cell identification, effector secreted pro-inflammatory cytokine, pro-apoptotic protease, ±×¸®°í cell count ¿Í cell membrane integrity ¸¦
µ¿½Ã¿¡ ÃøÁ¤ÀÌ °¡´ÉÇÑ Kit ÀÔ´Ï´Ù. |
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fluorescent membrane integrity dye ¸¦ ÀÌ¿ëÇÏ¿© »ì¾ÆÀÖ´Â ¼¼Æ÷¿Í Á×Àº¼¼Æ÷¸¦ ±¸º°ÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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fluorescent encoder dye ¸¦ ÀÌ¿ëÇÏ¿© Target Cell °ú Effector Cell À» ±¸º°ÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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CD3- CD56+ NK cells ¸¦ ±¸º°ÇÏ°Ô ÇÏ´Â fluorescent antibody panel À» ÀÌ¿ëÇÏ¿© NK Cell À» Immunophenotyping À» ÇÒ ¼ö ÀÖÀ¸¸ç ¶ÇÇÑ
NK Cell Ç¥¸é¿¡¼ IgG Fc receptor III (CD16) °¡ ¹ßÇöµÇ´Â Áö¸¦ È®ÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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fluorescent antibody panel ¿¡´Â 2°³ÀÇ ´Ù¸¥ ±â´ÉÀû È°¼º ¸¶Ä¿ÀÎ CD69 ¿Í CD25 ¸¦ Æ÷ÇÔÇÏ°í ÀÖ½À´Ï´Ù |
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°°Àº ÇϳªÀÇ Assay Well ¿¡¼ pro-inflammatory effector secreted cytokine IFN¥ã °ú pro-apoptotic serine protease Granzyme B À» µ¿½Ã¿¡ ÃøÁ¤ÇÒ ¼ö ÀÖµµ·Ï
½ÇÇèÀÇ È帧À» ÃÖÀûÈ ÇÏ¿´½À´Ï´Ù. |
Key Benefits | |
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Speed : ºü¸¥ ½Ã·á 󸮷κÎÅÍ ½ÇÁúÀûÀÎ µ¥ÀÌÅ͸¦ ¾òÀ½À¸·Î½á ÇÑ ¹ø¿¡ ¿©·¯ Á¾·ùÀÇ ½ÇÇèÀ» µ¿½Ã¿¡ ½ÇÇàÇÏ¿©, ¼ÕÀ¸·Î Á÷Á¢ ½ÇÇèÇÏ´Â ½Ã°£°ú ÃÑ ½ÇÇèȽ¼ö¸¦ ÁÙÀÌ°í,
¼ÒÁßÇÑ ½Ã·á¸¦ º¸Á¸ÇÏ´Â °ÍÀÌ °¡´ÉÇÕ´Ï´Ù |
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Results : single well assay ¸¦ ÅëÇØ activation markers (CD69 and CD25), cell membrane integrity, cell count,
secreted pro-inflammatory cytokine IFN¥ã, and pro-apoptotic protease Granzyme B ÀÇ ÃøÁ¤ÀÌ °¡´ÉÇÕ´Ï´Ù. |
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Biological insights : ÇϳªÀÇ plate well ¿¡ ÀÖ´Â ¼¼Æ÷ÀÇ phenotype °ú function À» ÇѲ¨¹ø¿¡ ºÐ¼®ÇÏ¿© µ¿ÀÏÇÑ ¼¼Æ÷¿¡¼
cell health ¸¦ µ¿½Ã¿¡ ºÐ¼®ÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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Ease of use : ÇϳªÀÇ platform ¿¡¼ assay ÁøÇàÀ» ÇÏ°í ¶ÇÇÑ °°Àº platform ¿¡ ÀÖ´Â iQue Forecyt¢ç ¼ÒÇÁÆ®¿þ¾î¸¦ ÀÌ¿ëÇÏ¿© µ¥ÀÌÅ͸¦ ºÐ¼®ÇϹǷÎ
¿©·¯ ¼Ò½º·Î ºÎÅÍ µ¥ÀÌÅ͸¦ ºÒ·¯¿À°í ºñ±³ÇÏ´Â º¹ÀâÇÑ °úÁ¤ÀÌ ¾øÀ¸¹Ç·Î ½Ã°£ÀÌ ¸Å¿ì Àý¾àµÇ°í Æí¸®ÇÕ´Ï´Ù |
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Flexibility : iQue¢ç Human NK Cell Companion Kits ¿¡ ÀÖ´Â °ËÁõµÈ ¸®½ºÆ®·Î ºÎÅÍ Ãß°¡ÀûÀÎ Cytokine ÃøÁ¤°ªÀ» ¼±ÅÃÇÏ¿© »ç¿ëÇÏ½Ç ¼ö ÀÖ½À´Ï´Ù. |
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ÇϳªÀÇ plate well ¿¡¼ target cell identification, cell health, cell function, immunophenotyping, ±×¸®°í cytokine profiling À» µ¿½Ã¿¡ ÃøÁ¤ÀÌ °¡´ÉÇÕ´Ï´Ù.
¶ÇÇÑ fluorescent encoding dye ¸¦ ÀÌ¿ëÇϸé Target Cell °ú Effector Cell À» ±¸ºÐÇÒ ¼ö ÀÖ½À´Ï´Ù. (encoding dye is not shown in this illustration) |
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Workflow of iQue¢ç Human NK Cell Killing Kit |
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