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¿µÁøÄÚÆÛ·¹ÀÌ¼Ç Á¾ÇÕÄ«´Ù·Î±× |
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iQue¢ç Human T Cell Memory Kit ´Â ¼¼Æ÷ÀÇ °¢ ´Ù¸¥ stage ¿¡ ÀÖ´Â T cell memory phenotype °ú ±× function À» ÃøÁ¤ÇÏ¸ç µ¿½Ã¿¡ Cell Health ¿Í cytokine secretion °ü·ÃÇÑ ±â´É¿¡ ´ëÇÑ Á¤º¸¸¦ Á¦°øÇÕ´Ï´Ù. |
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one washing ¸¸ ÇϹǷΠ½ÇÇè ÁøÇàÀÌ ¸Å¿ì °£ÆíÇÏ°í ¼¼Æ÷¿Í ºñµå¸¦ µ¿½Ã¿¡ ÃøÁ¤ÇϹǷΠ´ÙÀ½°ú °°Àº »ý¹°ÇÐÀû ÀÌÇظ¦ Çϴµ¥ ¸¹Àº µµ¿òÀ» Á¦°øÇÕ´Ï´Ù. |
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fluorescent membrane integrity dye ¸¦ ÀÌ¿ëÇÏ¿© »ì¾ÆÀÖ´Â ¸é¿ª¼¼Æ÷¸¸ ÃøÁ¤ÇÕ´Ï´Ù. |
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¼¼Æ÷µéÀ» fluorescent antibody panel ·Î ¿°»öÇÏ¿© CD3+ T cells, CD3- non-T cells, CD4+ T helper cells, CD8+ T cytotoxic cells µîÀ¸·Î ºÐ¸®ÇÏ¿© ¼¼Æ÷¸¦ phenotyping ÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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ÀÌ Kit ÀÇ Panel Àº T naive/memory/effector cell phenotyping À» À§ÇÑ 5°³ÀÇ ´Ù¸¥ Á¾·ùÀÇ T cell surface markers ¸¦ °¡Áö°í ÀÖ½À´Ï´Ù. (CD45RA, CD45RO, CD27 (long term survival), CD62L, and CD95) |
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ÃÖÀûÈµÈ ½ÇÇèÈ帧¿¡ ÀÇÇØ activated T cell ¿¡ ÀÇÇØ ºÐºñµÈ pro-inflammatory cytokine IFN¥ã and anti-inflammatory cytokine IL-10 °ú °°Àº effector cytokine À» ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù. |
Key Benefits | |
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Speed : ¿©·¯ Á¾·ùÀÇ assay ¸¦ µ¿ÀÏÇÑ well ¿¡¼ µ¿½Ã¿¡ ÁøÇà°¡´ÉÇÏ¿© ½Ã°£, ºñ¿ë ±×¸®°í ¼ÒÁßÇÑ ½Ã·áÀÇ »ç¿ëÀ» ÁÙÀÏ ¼ö ÀÖ½À´Ï´Ù. |
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Results : ÇϳªÀÇ assay well ¿¡¼ °¢±â ´Ù¸¥ stage ¿¡¼ÀÇ T cell memory phenotypes °ú function, ±×¸®°í cell viability, cell count, secreted pro/anti-inflammatory cytokines ¸¦
µ¿½Ã¿¡ ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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Biological insights : ÇϳªÀÇ plate well ¿¡ ÀÖ´Â ¼¼Æ÷¿Í cytokine À» µ¿½Ã¿¡ È®ÀÎÇÒ ¼ö ÀÖÀ¸¹Ç·Î µ¿ÀÏÇÑ ¼¼Æ÷ÀÇ Health ¿Í Function °ü·Ã µ¥ÀÌÅ͸¦ ±¸ÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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Simplicity : ÇϳªÀÇ platform ¿¡¼ ½ÇÇè°ú µ¥ÀÌÅÍ ºÐ¼®ÀÌ µ¿½Ã¿¡ ÁøÇàÇÒ ¼ö ÀÖÀ¸¹Ç·Î ½Ã°£À» ´ëÆø ÁÙÀÏ ¼ö ÀÖ½À´Ï´Ù. |
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ÇϳªÀÇ well ¿¡¼ T cell phenotypes, T memory subsets, cell health, secreted cytokines À» µ¿½Ã¿¡ ÃøÁ¤ÀÌ °¡´ÉÇÕ´Ï´Ù |
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The Workflow of iQue¢ç Human T Cell Memory Kit |
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