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¡ì Cell Health µ¹¾Æ°¡±â |
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iQue¢ç Cell Membrane Integrity Dyes ´Â membrane À» Åõ°úÇÒ ¼ö ¾ø´Â Reagents ·Î ±¸¼ºµÇ¾îÀÖÀ¸¸ç cell membrane integrity È®ÀÎÀ» ÅëÇØ Cell Viability ¸¦ ÃøÁ¤ÇÏ°Ô µË´Ï´Ù.
4°¡Áö ´Ù¸¥ ÆÄÀå¹üÀ§¸¦ °¡Áö°í ÀÖ´Â ¶Ç´Ù¸¥ iQue¢ç reagents ¿Í ÇÔ²² »ç¿ëÇÏ¸é ¿©·¯°³ Å×½ºÆ®¸¦ µ¿½Ã¿¡ ÁøÇàÇÒ ¼ö ÀÖ´Â iQue¢ç Cell Membrane Integrity Dyes ´Â
º°µµ·Î washing ÇÒ ÇÊ¿ä°¡ ¾ø°í, ¼¼Æ÷¿¡ ´ëÇÑ µ¶¼ºÀÌ °ÅÀÇ ¾øÀ¸¸ç, ÃÖ´ë 48½Ã°£ µ¿¾È Çü±¤ÀÌ ¾ÈÁ¤ÀûÀ¸·Î ¹ß»êµÇ´Â Ư¡À» °¡Áö°í ÀÖ´Ù.
B | Red reagent dye ´Â 4% paraformaldehyde (PFA) ¿¡¼ fixation ÇÑ ÈÄ¿¡µµ 18½Ã°£±îÁö Çü±¤À» ¹ß»ý½Ãų ¼ö ÀÖÀ¸¸ç HeLa ¿Í A459 ¿Í °°Àº ¼¼Æ÷ÁÖ¿¡µµ È¿À²ÀûÀ¸·Î Àß »ç¿ëµÇ°í ÀÖ´Ù. |
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iQue¢ç Cell Membrane Integrity Dye Assay ¿ø¸® :: °Ç°ÇÑ cell membrane À» °¡Áø ¼¼Æ÷´Â cell impermeable reagents ÀÇ Åõ°ú¸¦ ¸·¾Æ Çü±¤ÀÌ ¹ß»ýÀÌ µÇÁö ¾ÊÁö¸¸
membrane ÀÌ ¸Á°¡Áö°Ô µÇ¸é ±× reagent ´Â ¼¼Æ÷¸·À» Åë°úÇÏ¿© DNA ¿¡ binding ÇÏ°Ô µÇ¾î Çü±¤À» ¹ß»ý½ÃÅ°°Ô µÈ´Ù. |
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Example readouts to identify cell populations with intact membranes and compromised membranes |
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