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¿µÁøÄÚÆÛ·¹ÀÌ¼Ç Á¾ÇÕÄ«´Ù·Î±× |
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¡ì Cell Health µ¹¾Æ°¡±â |
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iQue¢ç Cell Cycle Kit ´Â DNA¿¡ »ðÀԵǴ Çü±¤ Dye ¸¦ »ç¿ëÇÏ¿© ¼¼Æ÷ÀÇ G0 | G1, G2 | M ¹× S ´Ü°è¸¦ ÃæºÐÈ÷ ±¸ºÐÇÏ¿© ÁÝ´Ï´Ù.
¶ÇÇÑ ÀÌ Kit ´Â ÀÏȸÀÇ 1½Ã°£ÀÇ ¼¼Æ÷ ¹è¾ç¸¸À¸·Îµµ °á°ú ºÐ¼®ÀÌ °¡´ÉÇϸç ÀϹÝÀûÀÎ ´Ù¸¥ ¹æ¹ý°ú ´Þ¸®
washing, RNase ó¸®, fixing °ú °°Àº ´Ü°è°¡ ÇÊ¿ä¾ø¾î ¸Å¿ì Æí¸®ÇÕ´Ï´Ù. |
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Determining cell cycle stage by DNA content |
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iQue¢ç Cell Cycle Kit ¸¦ ÀÌ¿ëÇÏ¿© cell cycle ÀúÇØÁ¦ÀÎ Nocodazole ¸¦ ó¸®ÇÏ¿´À» ¶§¿Í (gray histogram), ó¸®ÇÏÁö ¾Ê¾ÒÀ» ¶§¸¦ (yellow histogram) ¼·Î ºñ±³ÇÒ ¼ö ÀÖ½À´Ï´Ù.
¶ÇÇÑ °¢ cycle ´Ü°è (G0 | G1, G2 | M, and S) ¿¡ ÀÖ´Â ¼¼Æ÷ÀÇ ºñÀ²À» iQue Forecyt¢ç software ¿¡ ÀÖ´Â ±â´ÉÀÎ Heat Map À» ÀÌ¿ëÇÏ¿© ¼Õ½±°Ô ºñ±³ÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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