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¿µÁøÄÚÆÛ·¹ÀÌ¼Ç Á¾ÇÕÄ«´Ù·Î±× |
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¡ì Cell Health µ¹¾Æ°¡±â |
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washing ÀÌ ÇÊ¿äÇÏÁö ¾Ê´Â iQue¢ç Human 4-Plex Apoptosis Kit ´Â ÇϳªÀÇ sample ¿¡¼ Caspase 3/7 activation, Annexin V binding, cell viability, ±×¸®°í mitochondrial depolarization °á°ú¸¦
µ¿½Ã¿¡ È®ÀÎÀÌ °¡´ÉÇÏ¸ç ¶ÇÇÑ °úµµÇÑ µ¶¼º 󸮸¦ ½Äº°Çϱâ À§ÇØ ÃÑ ¼¼Æ÷ ¼öµµ È®ÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù.
ÃÑ 4°³ÀÇ reagents ¸¦ µ¿½Ã¿¡ »ç¿ëÇϰųª (A), ¶Ç´Â Çϳª¾¿ º°µµ·Îµµ »ç¿ëÀÌ °¡´ÉÇÕ´Ï´Ù.(B) |
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Principle of the iQue¢ç Human 4-Plex Apoptosis Kit.
BL1 : È¿¼Ò¿¡ ÀÇÇØ cleavage °¡ µÈ Caspase 3/7 ´Â È°¼ºÈµÇ¾î È®ÀÎÀ» ÇÒ ¼ö ÀÖ½À´Ï´Ù.
BL2 : ¼¼Æ÷¸· ¿ÜºÎ¿¡ ¹ßÇöµÈ phosphatidylserine Àº Annexin V ¿¡ ÀÇÇØ È®ÀÎÀÌ µË´Ï´Ù.
BL4 : membrane ÀÌ porous ÇÑ »óÅ°¡ µÇ¸é membrane impermeable dye °¡ ¼¼Æ÷¸·À» Åë°úÇÏ°Ô µÇ¾î Cell Viability ¸¦ ÃøÁ¤ÇÒ ¼ö ÀÖ°Ô µË´Ï´Ù.
RL1 : ¹ÌÅäÄܵ帮¾Æ lumen ³»ºÎ¿¡ À§Ä¡ÇÏ´Â Dye ¸¦ ÀÌ¿ëÇÏ¿© ¹ÌÅäÄܵ帮¾Æ ¼¼Æ÷¸·ÀÇ potential À» È®ÀÎÇÒ ¼ö ÀÖÀ¸¸ç ¹ÌÅäÄܵ帮¾Æ°¡ depolarization ÀÌ µÇ°ÔµÇ¸é
Dye °¡ ¼¼Æ÷Áú·Î ºüÁ®³ª°¡ ¹ÌÅäÄܵ帮¾Æ´Â Çü±¤»öÀ» ÀÒ°Ô µË´Ï´Ù. |
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(A) : cell death ÀÇ 4°¡Áö µ¶Æ¯ÇÑ Æ¯Â¡Àº Apoptosis Pathway È®ÀÎÀ» °¡´ÉÇÏ°Ô ÇÕ´Ï´Ù.
(B) : Staurosprorine, Mitomycin C, Methotrexate ±×¸®°í Dexamethasone ÈÇй°Áú¿¡ ´ëÇÑ Compound Dose Response Curves. |
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