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¡ì Antibody Characterization µ¹¾Æ°¡±â |
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(A) iQue¢ç Mouse IgG Type and Titer Kit assay ¿ø¸® :: secreted lgG ¿Í °¢±â ´Ù¸¥ Çü±¤À¸·Î labeling µÈ 4 isotype mouse igG ´Â four isotype specific IgG Capture Beads ¿¡ ´ëÇØ
°æÀïÀûÀ¸·Î binding À» ÇÏ°Ô µË´Ï´Ù. secreted lgG ÀÇ ³óµµ´Â FITC-lgG ¿¡ ÀÇÇÑ fluorescence signal ÀÇ ¼¼±â¿¡ ¹Ýºñ·ÊÇÏ°Ô µÇ¹Ç·Î secreted lgG ÀÇ ³óµµ¸¦ ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù
°¢ plate well ¿¡´Â membrane integrity ÃøÁ¤ Dye °¡ ÀÖ¾î ¼¼Æ÷ÀÇ cell viability ¸¦ ÃøÁ¤ÇÏ°Ô µË´Ï´Ù
(B) °¢ plate well ¿¡ ÀÖ´Â 4Á¾·ùÀÇ isotype-specific beads ¿¡ ´ëÇÑ °¢ isotype IgG ÀÇ concentration Àº standard curve ¸¦ ÅëÇØ ÃøÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù. |
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iQue Forecyt¢ç ÇÁ·Î±×·¥À» ÀÌ¿ëÇÏ¿© °¢ isotype igG ÀÇ »ý»ê È帧À» ½Ã°¢ÀûÀ¸·Î È®ÀÎÇÒ ¼ö ÀÖ´Â Heat Map À» ¸¸µé°Å³ª
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